Skip to main content
SpringerLink
Log in

Effect of naphthalene on photosystem 2 photochemical activity of pea plants

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

The effect of a typical polyaromatic hydrocarbon, naphthalene (Naph), on photosystem 2 (PS-2) photochemical activity in thylakoid membrane preparations and 20-day-old pea leaves was studied. Samples were incubated in water in the presence of Naph (0.078, 0.21, and 0.78 mM) for 0.5–24 h under white light illumination (15 μmol photons·m−2·s−1). The PS-2 activity was determined by studying fast and delayed chlorophyll (Chl) a fluorescence. Incubation of samples in water solutions at Naph concentrations of 0.21 and 0.78 mM led to a decrease in the maximum PS-2 quantum efficiency (Fv/Fm), noticeable changes in the polyphasic induction kinetics of fluorescence (OJIP), and a decrease in the amplitudes of the fast and slow components of delayed fluorescence of Chl a. The rate of release of electrolytes from leaves that were preliminarily incubated with Naph (0.21 mM) was also increased. Significant decrease in the fluorescence parameters in thylakoid membrane preparations was observed at Naph concentration of 0.03 mM and 12-min exposure of the samples. Chlorophyll (a and b) and carotenoid content (mg per gram wet mass) was insignificantly changed. The quantum yields of electron transfer from QA to QB (φET2o) and also to the PS-1 acceptors (φRE1o) were reduced. These results are explained by the increase in the number of QB-non-reducing centers of PS-2, which increased with increasing Naph concentration and exposure time of leaves in Naph solution. The suppression of PS-2 activity was partly abolished in the presence of the electron donor sodium ascorbate. Based on these results, it is suggested that Naph distorts cell membrane intactness and acts mainly on the PS-2 acceptor and to a lesser degree on the PS-2 donor side.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Asc:

sodium ascorbate

Chl:

chlorophyll

DFl:

delayed fluorescence

Fo :

minimum fluorescence

Fm :

maximum fluorescence

Fv :

variable fluorescence (Fv = Fo − Fm)

FFl:

fast fluorescence

Naph:

naphthalene

PA:

photosynthetic apparatus

PAHs:

polycyclic aromatic hydrocarbons

PS-2:

photosystem 2

QA :

primary quinone acceptor of PS-2

QB :

secondary quinone acceptor of PS-2

RC:

reaction center

TM:

thylakoid membranes

References

  1. Fang, G.-C., Wu, Y.-S., Chen, J.-C., Chang, C.-N., and Ho., T.-T. (2006) Characteristic of polycyclic aromatic hydrocarbon concentrations and source identification for fine and coarse particulates at Taichung Harbor near Taiwan Strait during 2004–2005, Sci. Total Environ., 366, 729–738.

    Article  CAS  PubMed  Google Scholar 

  2. Simonich, S., and Hites, R. A. (1994) Importance of vegetation in removing polycyclic aromatic hydrocarbons from the atmosphere, Nature, 370, 49–51.

    Article  CAS  Google Scholar 

  3. Chirkova, T. V. (2002) Physiological Basis of Plant Resistance [in Russian], St. Petersburg State University, St. Petersburg.

    Google Scholar 

  4. Kummerova, M., Krulova, J., Zezulka, S., and Triska, J. (2006) Evaluation of fluoranthene phytotoxicity in pea plants by Hill reaction and chlorophyll fluorescence, Chemosphere, 65, 489–496.

    Article  CAS  PubMed  Google Scholar 

  5. Kummerova, M., and Vanova, L. (2007) Chlorophyll fluorescence as an indicator of fluoranthene phototoxicity, Plant Soil Environ., 53, 430–436.

    Article  CAS  Google Scholar 

  6. Jajoo, A., Rao, M. N., Tomar, R. S., Grieco, M., Tikkanen, M., and Aro, E.-M. (2014) Inhibitory effects of polycyclic aromatic hydrocarbons (PAHs) on photosynthetic performance are not related to their aromaticity, J. Photochem. Photobiol. B, 135, 151–155.

    Article  Google Scholar 

  7. Bigler, W., and Schreiber, U. (1990) Chlorophyll luminescence as an indicator of stress-induced damage to the photosynthetic apparatus, Photosynth. Res., 25, 161–171.

    Article  Google Scholar 

  8. Stirbet, A., and Govindjee (2011) On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: basics and applications of the OJIP fluorescence transient, J. Photochem. Photobiol. B, 104, 36–57.

    Article  Google Scholar 

  9. Kalaji, H. M., Golstev, V., Bosa, K., Allakhverdiev, S. I., Strasser, R. J., and Govindjee (2012) Experimental in vivo measurements of light emission in plants: a perspective dedicated to David Walker, Photosynth. Res., 114, 69–96.

    Article  CAS  PubMed  Google Scholar 

  10. Strasser, R. J., Srivastava, A., and Tsimilli-Michael, M. (2000) in Probing Photosynthesis: Mechanisms, Regulation and Adaptation (Yunus, M., Pathre, U., and Mohanty, P., eds.) Taylor and Francis, London, pp. 445–483.

  11. Strasser, R. J., Tsimilli-Michael, M., and Srivastava, A. (2004) in Chlorophyll a Fluorescence: a Signature of Photosynthesis, Advances in Photosynthesis and Respiration (Papageorgiou, G. C., and Govindjee, eds.) Springer, Dordrecht, pp. 321–362.

    Google Scholar 

  12. Perlman, R. S., Yalkowsky, S. H., and Banerjee, S. (1984) Water solubilities of polynuclear aromatic and heteroaromatic compounds, J. Phys. Chem. Ref. Data, 13, 555–562.

    Article  Google Scholar 

  13. Kreslavski, V. D., Lankin, A. V., Vasilyeva, G. K., Lyubimov, V. Yu., Semenova, G. N., Schmitt, F.-J., Friedrich, T., and Allakhverdiev, S. I. (2014) Effects of polyaromatic hydrocarbons on photosystem II activity in pea leaves, Plant Physiol. Biochem., 81, 135–142.

    Article  CAS  PubMed  Google Scholar 

  14. Mehta, P., Kreslavsky, V., Bharti, S., Allakhverdiev, S. I., and Jajoo, A. (2011) Analysis of salt stress induced changes in photosystem II heterogeneity by prompt fluorescence and delayed fluorescence in wheat (Triticum aestivum) leaves, J. Photochem. Photobiol. B, 104, 308–313.

    Article  CAS  PubMed  Google Scholar 

  15. Lichtenthaler, H. K., and Wellburn, A. R. (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes, Methods Enzymol., 148, 350–382.

    Article  CAS  Google Scholar 

  16. Genty, B., Briantais, J-M., and Baker, N. R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence, Biochim. Biophys. Acta, 990, 87–92.

    Article  CAS  Google Scholar 

  17. Liu, H., Weisman, D., Ye, Y. B., Cui, B., Huang, Y. H., Colon-Carmona, A., and Wang, Z. H. (2009) An oxidative stress response to polycyclic aromatic hydrocarbon exposure is rapid and complex in Arabidopsis thaliana, Plant Sci., 176, 357–382.

    Google Scholar 

  18. Bukhov, N. G., and Carpentier, R. (2000) Heterogeneity of photosystem II reaction centers as influenced by heat treatment of barley leaves, Physiol. Plant., 110, 279–285.

    Article  CAS  Google Scholar 

  19. Singh-Tomar, R., and Jajoo, A. (2013) Alteration in PS II heterogeneity under the influence of polycyclic aromatic hydrocarbon (fluoranthene) in wheat leaves (Triticum aestivum), Plant Sci., 209, 58–63.

    Article  CAS  PubMed  Google Scholar 

  20. Jajoo, A., Mekala, N. R., Tomar, R. S., Grieco, M., Tikkanen, M., and Aro, E.-M. (2014) Inhibitory effects of polycyclic aromatic hydrocarbons (PAHs) on photosynthetic performance are not related to their aromaticity, J. Photochem. Photobiol. B, 137, 151–155.

    Article  CAS  PubMed  Google Scholar 

  21. Velthuys, B. R. (1981) Electron-dependent competition between plastoquinone and inhibitors for binding to photosystem II, FEBS Lett., 126, 277–281.

    Article  CAS  Google Scholar 

  22. Smirnoff, N., and Wheeler, G. L. (2000) Ascorbic acid in plants: biosynthesis and function, Crit. Rev. Plant Sci., 19, 267–290.

    Article  CAS  Google Scholar 

  23. Aksmann, A., and Tukaj, Z. (2008) Intact anthracene inhibits photosynthesis in algal cells: a fluorescence induction study on Chlamydomonas reinhardtii cw92 strain, Chemosphere, 74, 26–32.

    Article  CAS  PubMed  Google Scholar 

  24. Kummerova, M., Vanova, L., Krulova, J., and Zezulka, S. (2008) The use of physiological characteristics for comparison of organic compounds phytotoxicity, Chemosphere, 71, 2050–2059.

    Article  CAS  PubMed  Google Scholar 

  25. Van Gorkom, H. J. (1986) in Light Emission by Plants and Bacteria (Govindjee, Amesz, J., and Fork, D. C., eds.) Academic, Orlando, pp. 267–289.

  26. Rathod, D. P., Brestic, M., and Shao, H. B. (2011) Chlorophyll a fluorescence determines the drought resistance capabilities in two varieties of mycorrhized and nonmycorrhized Glycine max Linn, Afr. J. Microbiol. Res., 5, 4197–4206.

    Article  CAS  Google Scholar 

  27. Goltsev, V. N., Kuzmanova, M. A., Kaladzhi, H. M., and Allahverdiev, S. I. (2014) Variable and Delayed Fluorescence of Chlorophyll a Theoretical Basis and Practical Application in the Study of Plants [in Russian], Institute of Computer Science, Izhevsk-Moscow.

    Google Scholar 

  28. Goltsev, V., Zaharieva, I., Chernev, P., and Strasser, R. J. (2009) Delayed fluorescence in photosynthesis, Photosynth. Res., 101, 217–232.

    Article  CAS  PubMed  Google Scholar 

  29. Zhang, L., and Xing, D. (2008) Rapid determination of the damage to photosynthesis caused by salt and osmotic stresses using delayed fluorescence of chloroplasts, Photochem. Photobiol. Sci., 7, 352–360.

    Article  CAS  PubMed  Google Scholar 

  30. Chirkova, T. V. (1997) Cell membranes and plant resistance to stresses, Soros Obrazovat. Zh., 9, 12–17.

    Google Scholar 

  31. Duxbury, C. L., Dixon, D. G., and Greenberg, B. M. (1997) Effects of simulated solar radiation on the bioaccumulation of polycyclic aromatic hydrocarbons by the duckweed Lemna gibba, Environ. Toxicol. Chem., 16, 1739–1748.

    Article  CAS  Google Scholar 

  32. Murata, N., Takahashi, S., Nishiyama, Y., and Allakhverdiev, S. I. (2007) Photoinhibition of photosystem II under environmental stress, Biochim. Biophys. Acta, 1767, 414–421.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, 127276, Moscow, Russia

    A. V. Lankin, V. D. Kreslavski & S. I. Allakhverdiev

  2. Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, 142290, Pushchino, Moscow Region, Russia

    V. D. Kreslavski, S. K. Zharmukhamedov & S. I. Allakhverdiev

  3. Institute of Biological Engineering, Russian Academy of Sciences, ul. Institutskaya 7, 142290, Pushchino, Moscow Region, Russia

    A. Yu. Khudyakova

Authors
  1. A. V. Lankin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. D. Kreslavski
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Yu. Khudyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. K. Zharmukhamedov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. I. Allakhverdiev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to V. D. Kreslavski, S. K. Zharmukhamedov or S. I. Allakhverdiev.

Additional information

Published in Russian in Biokhimiya, 2014, Vol. 79, No. 11, pp. 1493–1504.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM14-177, September 7, 2014.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lankin, A.V., Kreslavski, V.D., Khudyakova, A.Y. et al. Effect of naphthalene on photosystem 2 photochemical activity of pea plants. Biochemistry Moscow 79, 1216–1225 (2014). https://doi.org/10.1134/S0006297914110091

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297914110091

Key words

Search

Navigation