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Heat shock induces production of reactive oxygen species and increases inner mitochondrial membrane potential in winter wheat cells

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Abstract

Heat shock leads to oxidative stress. Excessive ROS (reactive oxygen species) accumulation could be responsible for expression of genes of heat-shock proteins or for cell death. It is known that in isolated mammalian mitochondria high protonic potential on the inner membrane actuates the production of ROS. Changes in viability, ROS content, and mitochondrial membrane potential value have been studied in winter wheat (Triticum aestivum L.) cultured cells under heat treatment. Elevation of temperature to 37–50°C was found to induce elevated ROS generation and increased mitochondrial membrane potential, but it did not affect viability immediately after treatment. More severe heat exposure (55–60°C) was not accompanied by mitochondrial potential elevation and increased ROS production, but it led to instant cell death. A positive correlation between mitochondrial potential and ROS production was observed. Depolarization of the mitochondrial membrane by the protonophore CCCP inhibited ROS generation under the heating conditions. These data suggest that temperature elevation leads to mitochondrial membrane hyperpolarization in winter wheat cultured cells, which in turn causes the increased ROS production.

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Abbreviations

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

DCF:

2′,7′-dichlorofluorescein

FDA:

fluorescein diacetate

H2DCF·DA:

2′,7′-dichlorofluorescin diacetate

JC-1:

5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide

PI:

propidium iodide

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Division of the Russian Academy of Sciences, ul. Lermontova 132, 664033, Irkutsk, Russia

    A. V. Fedyaeva, A. V. Stepanov, I. V. Lyubushkina, T. P. Pobezhimova & E. G. Rikhvanov

  2. Irkutsk State University, ul. Suhe-Bator 5, 664003, Irkutsk, Russia

    I. V. Lyubushkina

Authors
  1. A. V. Fedyaeva
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  2. A. V. Stepanov
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  3. I. V. Lyubushkina
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  4. T. P. Pobezhimova
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  5. E. G. Rikhvanov
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Correspondence to A. V. Fedyaeva.

Additional information

Published in Russian in Biokhimiya, 2014, Vol. 79, No. 11, pp. 1476–1486.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM14-149, September 7, 2014.

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Fedyaeva, A.V., Stepanov, A.V., Lyubushkina, I.V. et al. Heat shock induces production of reactive oxygen species and increases inner mitochondrial membrane potential in winter wheat cells. Biochemistry Moscow 79, 1202–1210 (2014). https://doi.org/10.1134/S0006297914110078

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  • DOI: https://doi.org/10.1134/S0006297914110078

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