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Effect of detergents, trypsin, and bivalent metal ions on interfacial activation and functioning of phospholipase D

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Abstract

The effects of detergents, trypsin, and bivalent metal ions on production of phosphatidic and lysophosphatidic acids by the action of phospholipase D (PLD) on lecithin and lysolecithin were studied. It was found that these reaction products and dodecyl sulfate ions activate PLD, whereas other anionic detergents are less effective. A protective effect of the functioning enzyme against its hydrolytic inactivation by trypsin was found. Bivalent metal ions can be arranged in the following sequence by their ability to activate PLD in the hydrolysis of lecithin and lysolecithin: Ca2+ > Sr2+ > Ba2+ > Mg2+. These results are considered in relation to a proposed mechanism of activation and functioning of PLD with the participation of clusters of phosphatidates and lysophosphatidates. Such Me2+-induced formation of rafts or microdomains from the products of hydrolysis of phospholipids can rationalize not only PLD activation and self-regulation, but also the action of this mechanism on other components and properties of biomembranes. PLD and other lipolytic enzymes can be classified as lateral vector enzymes.

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Abbreviations

DG:

diacylglycerol

DOCA:

deoxycholic acid

FFA:

free fatty acid

LPA:

lysophosphatidic acid

LPC:

lysophosphatidylcholine

LPL:

lysophospholipids

Me2+ :

bivalent metal ions

PA:

phosphatidic acid

PC:

phosphatidylcholine

PL:

phospholipids

PLA2, PLC, PLD:

phospholipases A2, C, and D, respectively

SDS:

sodium dodecyl sulfate

TCA:

taurocholic acid

References

  1. Wait, M. (1987) The Phospholipases, Plenum Press, N.Y.-London.

    Book  Google Scholar 

  2. Foster, D. A., and Xu, L. (2003) Phospholipase D in cell proliferation and cancer, Mol. Cancer Res., 11, 789–800.

    Google Scholar 

  3. Donaldson, J. G. (2009) Phospholipase D in endocytosis and endosomal recycling pathways, Biochim. Biophys. Acta, 1791, 845–849.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Rudge, S. A., and Wakelam, M. J. (2009) Inter-regulatory dynamics of PLD and the actin cytoskeleton, Biochim. Biophys. Acta, 1791, 856–861.

    Article  CAS  PubMed  Google Scholar 

  5. Kanaho, Y., Funakoshi, Y., and Hasegawa, H. (2009) PLD signaling and its involvement in neurite outgrowth, Biochim. Biophys. Acta, 1791, 898–904.

    Article  CAS  PubMed  Google Scholar 

  6. Liscovitch, M., Czarny, M., Fiucci, G., and Tang, X. (2000) Phospholipase D: molecular and cell biology of a novel gene family, Biochem. J., 345, 401–415.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Rakhimov, M. M., and Madyarov, Sh. R. (1982) Role of phase states of phospholipids in regulation of catalytic effects of phospholipases in artificial and membrane systems, in Transport of Ions through Biological Membranes and Mechanism of Action of Biologically Active Compounds [in Russian] (Tashmukhamedov, B. A., ed.) FAN Publishers, Tashkent, pp. 186–230.

    Google Scholar 

  8. Szumilo, M., and Rahden-Staron, I. (2006) PLD in mammalian cells; structure, properties and physiological role, Postepy Hig. Med. Dosw., 60, 421–430.

    Google Scholar 

  9. Rakhimov, M. M., Kalendareva, T. N., Rashidova, S. Sh., and Madyarov, Sh. R. (1982) The role of calcium ions in the catalytic activity of phospholipase D, Biokhimiya, 47, 1649–1662.

    CAS  Google Scholar 

  10. Madyarov, Sh. R., and Rakhimov, M. M. (1989) Some peculiarities of water-soluble phospholipase D function in various substrate systems, Biokhimiya, 54, 1780–1789.

    CAS  Google Scholar 

  11. Madyarov, Sh. R. (2012) Isolation, purification, stabilization and characterization of phospholipase D preparations from Raphanus sativus longipinnatus, Khim. Prirod. Soedin., 4, 499–501.

    Google Scholar 

  12. Khole, V., Khole, V., and Madyarov, Sh. R. (1983) On interaction of cobra venom polypeptides with different substrate systems, Ind. J. Biochem. Biophys., 20, 177–179.

    CAS  Google Scholar 

  13. Ito, T., and Ohnishi, S.-I. (1974) Ca2+-induced lateral phase separation in PA-PC membranes, Biochim. Biophys. Acta, 352, 29–37.

    Article  CAS  PubMed  Google Scholar 

  14. Madyarov, Sh. R. (2002) Phospholipase D as vector controlling a membrane anisotropy, in Proc 43rd Int. Conf. Biochemistry of Lipids, ICBL, Graz, Austria, PO 130, p. 76.

    Google Scholar 

  15. Mad’yarov, Sh. R. (2004) Phospholipids clustering mode of phospholipase D action, in Abstr. 5th Int. Congr. on Biological Physics, ICBP, Gotheburg, Sweden, Aug. 23–27, B 05-206, p. 101.

    Google Scholar 

  16. Leiros, I., Secundo, F., Zambonelli, C., Servi, S., and Hough, E. (2000) The first crystal structure of phospholipase D, Structure, 8, 655–667.

    Article  CAS  PubMed  Google Scholar 

  17. Tinker, D. O., and Wei, J. (1979) Heterogeneous catalysis of phospholipase A2: formulation of a kinetic description of surface effects, Can. J. Biochem., 57, 97–106.

    Article  CAS  PubMed  Google Scholar 

  18. Jain, M. K., and Gelb, M. H. (1991) Phospholipase A2 catalyzed hydrolysis of vesicles: uses of interfacial catalysis in the scooting mode, Methods Enzymol., 197, 112–125.

    Article  CAS  PubMed  Google Scholar 

  19. Kravtsov, A. V., and Alekseenko, I. R. (1990) Mechanisms of Regulation of Biomembranes Vector Enzymes [in Russian], Naukova Dumka, Kiev.

    Google Scholar 

  20. Frieden, C. (1970) Kinetic aspects of regulation of metabolic processes. The hysteretic enzyme concept, J. Biol. Chem., 245, 5788–5799.

    CAS  PubMed  Google Scholar 

  21. Pieterson, W. A., Volwerk, J. J., and de Haas, G. H. (1974) Interaction of phospholipase A2 and its zymogen with divalent metal ions, Biochemistry, 13, 1439–1445.

    Article  CAS  PubMed  Google Scholar 

  22. Wells, M. A. (1974) The mechanism of interfacial activation of phospholipase A2, Biochemistry, 13, 2248–2257.

    Article  CAS  PubMed  Google Scholar 

  23. Wells, M. A. (1974) A phospholipase A2 model system. Calcium enhancement of the amine-catalyzed methanolysis of phosphatidylcholine, Biochemistry, 13, 2258–2264.

    Article  CAS  PubMed  Google Scholar 

  24. Taniguchi, Y., Ohba, T., Miyata, H., and Ohki, K. (2006) Rapid phase change of lipid microdomains in giant vesicles induced by conversion of sphingomyelin to ceramide, Biochim. Biophys. Acta, 1758, 145–153.

    Article  CAS  PubMed  Google Scholar 

  25. Bogdanov, M., Umeda, M., and Dowhan, W. (1999) Phospholipid-assisted refolding of an integral membrane protein. Minimum structural features for phosphatidylethanolamine to act as a molecular chaperone, J. Biol. Chem., 274, 12339–12345.

    Article  CAS  PubMed  Google Scholar 

  26. Madyarov, Sh. R., and Chung, B. H. (2001) Sensorial properties of phospholipase D in reflection of physicochemical states of membrane-like structures, in Proc. 4th Int. Congr. on Biological Physics, ICBP, July 30–August 3, Kyoto, Japan, p. 81.

    Google Scholar 

  27. Mokhova, E. N., Starkov, A. A., and Bobyleva, V. A. (1993) Oxidative phosphorylation uncoupling by fatty acids in liver and muscle mitochondria, Biochemistry (Moscow), 58, 1105–1112.

    Google Scholar 

  28. Nakamura, M. T., Yudell, B. E., and Loor, J. J. (2013) Regulation of energy metabolism by long-chain fatty acids, Prog. Lipid Res., 53, 124–144.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of the Gene Pool of Plants and Animals, Uzbek Academy of Sciences, 32 Bogi-shamol str., 101125, Tashkent, Uzbekistan

    Sh. R. Madyarov

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Correspondence to Sh. R. Madyarov.

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Original Russian Text © Sh. R. Madyarov, 2014, published in Biokhimiya, 2014, Vol. 79, No. 7, pp. 864–872.

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Madyarov, S.R. Effect of detergents, trypsin, and bivalent metal ions on interfacial activation and functioning of phospholipase D. Biochemistry Moscow 79, 687–693 (2014). https://doi.org/10.1134/S0006297914070104

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  • DOI: https://doi.org/10.1134/S0006297914070104

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