Abstract
Ribosomal protein L4 is a regulator of protein synthesis in the Escherichia coli S10 operon, which contains genes of 11 ribosomal proteins. In this work, we have investigated regulatory functions of ribosomal protein L4 of the thermophilic archaea Methanococcus jannaschii. The S10-like operon from M. jannaschii encodes not 11, but only five ribosomal proteins (L3, L4, L23, L2, S19), and the first protein is L3 instead of S10. We have shown that MjaL4 and its mutant form lacking an elongated loop specifically inhibit expression of the first gene of the S10-like operon from the same organism in a coupled transcription?translation system in vitro. By deletion analysis, an L4-binding regulatory site has been found on MjaL3 mRNA, and a fragment of mRNA with length of 40 nucleotides has been prepared that is necessary and sufficient for the specific interaction with the MjaL4 protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nomura, M., Yates, J. L., Dean, D., and Post, L. E. (1980) Proc. Natl. Acad. Sci. USA, 77, 7084–7088.
Zengel, J. M., and Lindahl, L. (1994) Prog. Nucleic Acids Res. Mol. Biol., 47, 331–370.
Zengel, J. M., and Lindahl, L. (1990) J. Mol. Biol., 213, 67–78.
Schuwirth, B. S., Borovinskaya, M. A., Hau, C. W., Zhang, W., Vila-Sanjurjo, A., Holton, J. M., and Cate, J. H. (2005) Science, 310, 827–834.
Tumminia, S. J., Hellmann, W., Wall, J. S., and Boublik, M. (1994) J. Mol. Biol., 235, 1239–1250.
Ban, N., Nissen, P., Hansen, J., Capel, M., Moore, P. B., and Steitz, T. A. (1999) Nature, 400, 841–847.
Timsit, Y., Acosta, Z., Allemand, F., Chiaruttini, C., and Springer, M. (2009) Int. J. Mol. Sci., 10, 817–834.
Zengel, J. M., Jerauld, A., Walke, A., Markus, R., Wahl, C., and Lindahl, L. (2003) RNA, 9, 1188–1197.
Shen, P., Zengel, J. M., and Lindahl, L. (1988) Nucleic Acids Res., 16, 8905–8924.
Stelzl, U., Zengel, J. M., Tovbina, M., Walker, M., Nierhaus, K. H., Lindahl, L., and Patel, D. J. (2003) J. Biol. Chem., 278, 28237–28245.
Stelzl, U., and Nierhaus, K. H. (2001) RNA, 7, 598–609.
Allen, T., Shen, P., Samsel, L., Liu, R., Lindahl, L., and Zengel, J. M. (1999) J. Bacteriol., 181, 6124–6132.
Studier, F., Rosenberg, A., Dunn, J., and Dubendorff, J. (1990) J. Methods Enzymol., 185, 60–89.
Tishchenko, S., Gabdulkhakov, A., Nevskaya, N., Sarskikh, A., Kostareva, O., Nikonova, E., Sycheva, A., Moshkovskii, S., Garber, M., and Nikonov, S. (2012) Acta Crystallogr. D, 68, 1051–1057.
Draper, D. E., Deckman, I. C., and Vartikar, J. V. (1988) Methods Enzymol., 164, 203–220.
Mayer, C., Kohrer, C., Grobner, P., and Piendl, W. (1998) Mol. Microbiol., 27, 455–468.
Kraft, A., Lutz, C., Lingenhel, A., Grobner, P., and Piendl, W. (1999) Genetics, 152, 1363–1372.
Amils, R., Cammarano, P., and Londei, P. (1993) in The Biochemistry of Archaea (Archaebacteria) (Kates, M., Kushner, D. J., and Matheson, A. T., eds.) Elsevier Biomedical Press, Amsterdam, pp. 393–438.
Zengel, J. M., Mueckl, D., and Lindahl, L. (1980) Cell, 21, 523–535.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A. O. Mikhaylina, O. S. Kostareva, A. V. Sarskikh, R. V. Fedorov, W. Piendl, M. B. Garber, S. V. Tishchenko, 2014, published in Biokhimiya, 2014, Vol. 79, No. 1, pp. 87–95.
Rights and permissions
About this article
Cite this article
Mikhaylina, A.O., Kostareva, O.S., Sarskikh, A.V. et al. Investigation of the regulatory function of archaeal ribosomal protein L4. Biochemistry Moscow 79, 69–76 (2014). https://doi.org/10.1134/S0006297914010106
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297914010106