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Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein

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Abstract

The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.

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References

  1. Ohlsson, R., Renkawitz, R., and Lobanenkov, V. (2001) Trends Genet., 17, 520–527.

    Article  PubMed  CAS  Google Scholar 

  2. Filippova, G. N. (2008) Curr. Top. Dev. Biol., 80, 337–360.

    Article  PubMed  CAS  Google Scholar 

  3. Nikolaev, L. G., Akopov, S. B., Didych, D. A., and Sverdlov, E. D. (2009) Curr. Genom., 10, 294–302.

    Article  CAS  Google Scholar 

  4. Filippova, G. N., Fagerlie, S., Klenova, E. M., Myers, C., Dehner, Y., Goodwin, G., Neiman, P. E., Collins, S. J., and Lobanenkov, V. V. (1996) Mol. Cell Biol., 16, 2802–2813.

    PubMed  CAS  Google Scholar 

  5. Klenova, E. M., Nicolas, R. H., Paterson, H. F., Carne, A. F., Heath, C. M., Goodwin, G. H., Neiman, P. E., and Lobanenkov, V. V. (1993) Mol. Cell Biol., 13, 7612–7624.

    PubMed  CAS  Google Scholar 

  6. Klenova, E. M., Nicolas, R. H. U. S., Carne, A. F., Lee, R. E., Lobanenkov, V. V., and Goodwin, G. H. (1997) Nucleic Acids Res., 25, 466–474.

    Article  PubMed  CAS  Google Scholar 

  7. Jiang, L., Qin, Y., Sun, Z. L., Wu, J., Yang, W. L., and Zhang, J. Z. (2009) Sichuan Da Xue Xue Bao Yi Xue Ban, 40, 1–5.

    PubMed  Google Scholar 

  8. Chernukhin, I. V., Shamsuddin, S., Robinson, A. F., Carne, A. F., Paul, A., El-Kady, A. I., Lobanenkov, V. V., and Klenova, E. M. (2000) J. Biol. Chem., 275, 29915–29921.

    Article  PubMed  CAS  Google Scholar 

  9. Chau, C. M., Zhang, X. Y., McMahon, S. B., and Lieberman, P. M. (2006) J. Virol., 80, 5723–5732.

    Article  PubMed  CAS  Google Scholar 

  10. Gluzman, Y. (1981) Cell, 23, 175–182.

    Article  PubMed  CAS  Google Scholar 

  11. Laemmli, U. K. (1970) Nature, 227, 680–685.

    Article  PubMed  CAS  Google Scholar 

  12. Merril, C. R., Dunau, M. L., and Goldman, D. (1981) Anal. Biochem., 110, 201–207.

    Article  PubMed  CAS  Google Scholar 

  13. Bell, A. C., West, A. G., and Felsenfeld, G. (1999) Cell, 98, 387–396.

    Article  PubMed  CAS  Google Scholar 

  14. Chung, J. H., Bell, A. C., and Felsenfeld, G. (1997) Proc. Natl. Acad. Sci. USA, 94, 575–580.

    Article  PubMed  CAS  Google Scholar 

  15. Baniahmad, A., Steiner, C., Kohne, A. C., and Renkawitz, R. (1990) Cell, 61, 505–514.

    Article  PubMed  CAS  Google Scholar 

  16. Kohne, A. C., Baniahmad, A., and Renkawitz, R. (1993) J. Mol. Biol., 232, 747–755.

    Article  PubMed  CAS  Google Scholar 

  17. Klochkov, D., Rincon-Arano, H., Ioudinkova, E. S., Valadez-Graham, V., Gavrilov, A., Recillas-Targa, F., and Razin, S. V. (2006) Mol. Cell Biol., 26, 1589–1597.

    Article  PubMed  CAS  Google Scholar 

  18. Vetchinova, A. S., Akopov, S. B., Chernov, I. P., Nikolaev, L. G., and Sverdlov, E. D. (2006) Anal. Biochem., 354, 85–93.

    Article  PubMed  CAS  Google Scholar 

  19. Kozak, M. (1987) Nucleic Acids Res., 15, 8125–8148.

    Article  PubMed  CAS  Google Scholar 

  20. Dunn, K. L., Zhao, H., and Davie, J. R. (2003) Exp. Cell Res., 288, 218–223.

    Article  PubMed  CAS  Google Scholar 

  21. Arnold, R., Burcin, M., Kaiser, B., Muller, M., and Renkawitz, R. (1996) Nucleic Acids Res., 24, 2640–2647.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    E. S. Kotova, I. V. Sorokina, S. B. Akopov, L. G. Nikolaev & E. D. Sverdlov

Authors
  1. E. S. Kotova
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  2. I. V. Sorokina
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  3. S. B. Akopov
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  4. L. G. Nikolaev
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  5. E. D. Sverdlov
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Corresponding author

Correspondence to S. B. Akopov.

Additional information

Published in Russian in Biokhimiya, 2013, Vol. 78, No. 8, pp. 1122–1127.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM13-106, June 23, 2013.

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Kotova, E.S., Sorokina, I.V., Akopov, S.B. et al. Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein. Biochemistry Moscow 78, 879–883 (2013). https://doi.org/10.1134/S0006297913080038

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  • DOI: https://doi.org/10.1134/S0006297913080038

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