Abstract
We have found that extracellular phosphomannan is the main phosphate reserve in the yeast Kuraishia capsulata, in contrast to other yeast species effectively absorbing Pi. Under nitrogen starvation, K. capsulata absorbed essentially all Pi from the medium containing 240 mM glucose, 2.5 mM MgSO4, and 11 mM KH2PO4. Inorganic polyphosphate level in the cells was about 14% of the Pi absorbed. Most of the Pi (∼60%) was found in the fraction of extracellular phosphomannan that can be used as a carbon and phosphorus source by this yeast in deficient media.
Similar content being viewed by others
References
Smirnov, A., Suzina, N., Chudinova, N., Kulakovskaya, T., and Kulaev, I. (2005) FEMS Microbiol. Ecol., 52, 129–137.
Ryazanova, L. P., Smirnov, A. V., Kulakovskaya, T. V., and Kulaev, I. S. (2007) Microbiology, 76, 663–669.
Ryazanova, L. P., Suzina, N. E., Kulakovskaya, T. V., and Kulaev, I. S. (2009) Arch. Microbiol., 191, 467–471.
Kulaev, I. S., Vagabov, V. M., and Kulakovskaya, T. V. (2004) The Biochemistry of Inorganic Polyphosphates, Wiley, Chichester.
Vagabov, V. M., Trilisenko, L. V., and Kulaev, I. S. (2000) Biochemistry (Moscow), 65, 349–354.
Rubin, G. M. (1973) J. Biol. Chem., 11, 3860–3875.
Cawley, T. N., Harrington, M. G., and Letters, R. (1972) Biochem. J., 129, 711–720.
Lichko, L., Kulakovskaya, T., Pestov, N., and Kulaev, I. (2006) Biosci. Rep., 26, 45–54.
Breus, N. A., Ryazanova, L. P., Dmitriev, V. V., Kulakovskaya, T. V., and Kulaev, I. S. (2012) FEMS Yeast Res., 12, 617–624.
Slodki, M. E., Wickerham, L. J., and Cadmus, M. C. (1961) J. Bacteriol., 82, 269–274.
Avigad, G., and Kalina, M. (1979) FEMS Microbiol. Lett., 6, 111–114.
Ferro, V., Fewings, K., Palermo, M. C., and Li, C. (2001) Carbohydr. Res., 332, 183–189.
Shimotoyodome, A., Kobayashi, H., Nakamura, J., Tokimitsu, I., Hase, T., Inoue, T., Matsukubo, T., and Takaesu, Y. (2006) Biofouling, 22, 261–268.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Biokhimiya, 2013, Vol. 78, No. 6, pp. 867–871.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM13-052, April 7, 2013.
Rights and permissions
About this article
Cite this article
Lichko, L.P., Kulakovskaya, T.V. & Kulaev, I.S. Extracellular phosphomannan as a phosphate reserve in the yeast Kuraishia capsulata . Biochemistry Moscow 78, 674–677 (2013). https://doi.org/10.1134/S0006297913060138
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297913060138