Biochemistry (Moscow)

, Volume 78, Issue 5, pp 485–495 | Cite as

Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds

Article

Abstract

Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two full-length cDNAs JcDHN-1 and JcDHN-2 encoding two dehydrins from Jatropha curcas seeds were identified and characterized. JcDHN-1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN-1 protein has 175 a.a. residues that form a 19.3-kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN-2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN-2 protein has 156 a.a. residues that form a 17.1-kDa polypeptide with a predicted pI of 7.09. JcDHN-1 is classified as type Y3SK2 and JcDHN-2 is classified as type Y2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indicates that both JcDHN-1 and JcDHN-2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN-2 has glycoside hydrolase GH20 super-family activity. Quantitative real time PCR analysis for JcDHN-1 and JcDHN-2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN-2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.

Key words

dehydrin Jatropha curcas real time PCR seed development 

Abbreviations

DAF

days after flowering

DHNs

dehydrins

DW

dry weight

FW

fresh weight

LEA

late embryogenesis abundant

qRT-PCR

real time quantitative reverse transcription-polymerase chain reaction

RACE

Rapid Amplification of cDNA Ends

RT-PCR

reverse transcription-polymerase chain reaction

WC

water content

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  1. 1.Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunChina
  2. 2.Genetic Department, Faculty of AgricultureTanta UniversityTantaEgypt
  3. 3.Agricultural Botany Department, Faculty of AgricultureTanta UniversityTantaEgypt
  4. 4.Department of Plant PhysiologyWarsaw University of Life Sciences SGGWWarsawPoland
  5. 5.Biology Department, Faculty of ScienceTaibah UniversityAlmadinah AlmunawwarahKSA (Saudi Arabia)
  6. 6.Botany Department, Faculty of ScienceTanta UniversityTantaEgypt
  7. 7.Department of Molecular BiologyPusan National UniversityBusanKorea
  8. 8.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  9. 9.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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