Abstract
The light-induced functioning of photosystem 2 (PS 2) is directly linked to the translocation of both electrons and protons across the membrane, which results in the formation of transmembrane electric potential difference (ΔΨ). Generation of ΔΨ due to S-state transitions of the water oxidation complex was demonstrated for the first time in Mn-depleted and reconstituted PS 2 core complexes incorporated into liposomes. The kinetics and relative amplitudes of the electrogenic reactions in dark-adapted samples during S1→S2, S2→S3, and S4→S0 transitions in response to the first, second and third laser flashes were comparable to those obtained in the intact PS 2 core particles. These results expand current understanding of the nature and mechanisms of electrogenic (vectorial) reactions due to a charge transfer on the donor side of PS 2.
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Abbreviations
- PS 2:
-
photosystem 2
- P680 :
-
primary electron donor
- QA and QB :
-
primary and secondary quinone acceptors, respectively
- RC:
-
reaction center
- WOC:
-
water oxidation complex
- YZ and Y ·z :
-
reduced and neutral radical form of Tyr161 residue of D1 protein, respectively
- ΔΨ:
-
transmembrane electric potential difference
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Published in Russian in Biokhimiya, 2013, Vol. 78, No. 4, pp. 513–521.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM12-328, February 3, 2013.
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Petrova, I.O., Kurashov, V.N., Zaspa, A.A. et al. Vectorial charge transfer reactions on the donor side of manganese-depleted and reconstituted photosystem 2 core complexes. Biochemistry Moscow 78, 395–402 (2013). https://doi.org/10.1134/S0006297913040081
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DOI: https://doi.org/10.1134/S0006297913040081