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Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria

Biochemistry (Moscow) volume 77, pages 362–371 (2012)Cite this article

Abstract

Artificial generation of oxygen superoxide radicals in actively growing cultures of Mycobacterium tuberculosis, Myc. smegmatis, and Corynebacterium ammoniagenes is followed by accumulation in the bacterial cells of substantial amounts of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcDP) — an intermediate of the non-mevalonate pathway of isoprenoid biosynthesis (MEP) — most possibly due to the interaction of the oxygen radicals with the 4Fe-4S group in the active center and inhibition of the enzyme (E)-4-oxy-3-methylbut-2-enyl diphosphate synthase (IspG). Cadmium ions known to inhibit IspG enzyme in chloroplasts (Rivasseau, C., Seemann, M., Boisson, A. M., Streb, P., Gout, E., Douce, R., Rohmer, M., and Bligny, R. (2009) Plant Cell Environ., 32, 82–92), when added to culture of Myc. smegmatis, substantially increase accumulation of MEcDP induced by oxidative stress with no accumulation of other organic phosphate intermediates in the cell. Corynebacterium ammoniagenes, well-known for its ability to synthesize large amounts of MEcDP, was also shown to accumulate this unique cyclodiphosphate in actively growing culture when NO at low concentration is artificially generated in the medium. A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed.

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Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071, Moscow, Russia

    V. Yu. Artsatbanov, G. N. Vostroknutova, M. O. Shleeva, A. V. Goncharenko, D. N. Ostrovsky & A. S. Kapreliants

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia

    A. I. Zinin

Authors
  1. V. Yu. Artsatbanov
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  2. G. N. Vostroknutova
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  3. M. O. Shleeva
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  4. A. V. Goncharenko
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  5. A. I. Zinin
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  6. D. N. Ostrovsky
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  7. A. S. Kapreliants
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Correspondence to D. N. Ostrovsky.

Additional information

Published in Russian in Biokhimiya, 2012, Vol. 77, No. 4, pp. 461–473.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM11-313, February 19, 2012.

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Artsatbanov, V.Y., Vostroknutova, G.N., Shleeva, M.O. et al. Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria. Biochemistry Moscow 77, 362–371 (2012). https://doi.org/10.1134/S0006297912040074

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  • DOI: https://doi.org/10.1134/S0006297912040074

Key words

  • non-mevalonate pathway
  • nitrosative stress
  • mycobacteria