Abstract
The type II bifunctional DNA methyltransferase (MTase) Ecl18 that is able to control transcription of its own gene was studied kinetically. Based on initial velocity dependences from S-adenosyl-L-methionine (AdoMet) and target DNA and substrate preincubation assays, it is proposed that the enzyme apparently works by a rapid equilibrium ordered bi-bi mechanism with DNA binding first. By measuring the enzyme activity depending on DNA and AdoMet at different fixed concentrations of the operator sequence oligonucleotide, it was found that its binding has noncompetitive inhibitory effect on Ecl18 MTase activity.
Similar content being viewed by others
Abbreviations
- AdoMet:
-
S-adenosyl-L-methionine
- DE:
-
diethylaminoethyl
- MTases:
-
DNA methyltransferases
- RE:
-
restriction endonuclease
References
Wilson, G. G. (1991) Nucleic Acids Res., 19, 2539–2565.
Beletskaya, I., Zakharova, M., Shlyapnikov, M., Semenova, L., and Solonin, A. (2000) Nucleic Acids Res., 28, 3817–3822.
Zakharova, M., Minakhin, L., Solonin, A., and Severinov, K. (2004) J. Mol. Biol., 335, 103–111.
Butler, D., and Fitzgerald, G. F. (2001) J. Bacteriol., 183, 4668–4673.
Som, S., and Friedman, S. (1993) EMBO J., 12, 4297–4303.
Som, S., and Friedman, S. (1997) J. Bacteriol., 179, 964–967.
Karyagina, A., Shilov, I., Tashlitskii, V., Khodoun, M., Vasil’ev, S., Lau, P., and Nikolskaya, I. (1997) Nucleic Acids Res., 25, 2114–2120.
Denjmukhametov, M., Brevnov, M., Zakharova, M., Repyk, A., Solonin, A., Petrauskene, O., and Gromova, E. (1998) FEBS Lett., 21, 233–236.
Protsenko, A., Zakharova, M., Nagornykh, M., Solonin, A., and Severinov, K. (2009) Nucleic Acids Res., 37, 5322–5330.
Wu, J., and Santi, D. (1987) J. Biol. Chem., 262, 4778–4786.
Lindstorm, J., Flynn, J., and Reich, N. (2000) J. Biol. Chem., 275, 4912–4919.
Bhattacharya, S., and Dubey, A. (1999) J. Biol. Chem., 274, 14743–14749.
Flynn, J., and Reich, N. (1998) Biochemistry, 37, 15162–15169.
Bacolla, A., Pradhan, S., Roberts, R., and Wells, R. (1999) J. Biol. Chem., 274, 33011–33019.
Yokochi, T., and Robertson, K. (2002) J. Biol. Chem., 277, 11735–11745.
Nikitin, D., Mokrishcheva, M., and Solonin, A. (2007) Biochim. Biophys. Acta, 1774, 1014–1019.
Roberts, D. V. (1977) in Enzyme Kinetics, Cambridge University Press, London-New York.
Bheemanaik, S., Chandrashekaran, S., Nagaraja, V., and Rao, D. (2002) J. Biol. Chem., 278, 7863–7874.
Kang, Y., Lee, H., Noh, M., Cho, N., and Yoo, O. (1995) Biochem. Biophys. Res. Commun., 206, 997–1002.
Reich, N., and Mashhoon, N. (1991) Biochemistry, 30, 2933–2939.
Romanenkov, A., Kisil, O., Zatsepin, T., Yamskova, O., Karyagina, A., Metelev, V., Oretskaya, T., and Kubareva, E. (2006) Biochemistry (Moscow), 71, 1341–1349.
Fedotova, E., Protsenko, A., Zakharova, M., Lavrova, N., Alekseevsky, A., Oretskaya, T., Karyagina, A., Solonin, A., and Kubareva, E. (2009) Biochemistry (Moscow), 74, 85–91.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Biokhimiya, 2012, Vol. 77, No. 3, pp. 392–397.
Rights and permissions
About this article
Cite this article
Nikitin, D.V., Mokrishcheva, M.L. & Solonin, A.S. Binding of DNA methyltransferase M.Ecl18 to operator-promoter region decreases its methylating activity. Biochemistry Moscow 77, 307–311 (2012). https://doi.org/10.1134/S0006297912030108
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297912030108