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Identification and secondary structure analysis of a keratin-like fibrous protein discovered in ligament of the bivalve Siliqua radiata

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Abstract

A novel keratin-like fibrous protein K58 with molecular weight of about 58 kDa was discovered in bivalve Siliqua radiata ligament and identified by amino acid composition and MALDI-TOF-TOF analysis. We found that the protein is composed of cylindrical fibers (∼160 nm in diameter) and contains high glycine (27.4%) and phenylalanine (10.5%) contents. Furthermore, it is homologous to keratin type II cytoskeletal 1, with repeat motifs of SGGG and SYGSGG. FTIR and secondary structure analysis indicate that K58 is composed of 46.2% β-sheet, 33.4% β-turn, 13.1% α-helix, and 4.7% disordered structure. This structure feature is closely related to the superior tensile strength, elasticity, and solvent resistance property of K58. These discoveries provide some evidence for evolution of keratin and fibrous proteins and prompt further studies of ligament fibrous proteins.

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References

  1. Trueman, E. R. (1950) Q. J. Micro. Sci., 91, 225–234.

    Google Scholar 

  2. Sartori, A. F., and Ball, A. D. (2009) J. Mollusc. Stud., 75, 295–304.

    Article  Google Scholar 

  3. Kahler, G. A., Sass, R. L., and Fisher, F. M. (1976) J. Comp. Physiol., 109, 209–220.

    CAS  Google Scholar 

  4. Beedham, G. E. (1958) Quart. J. Micro. Sci., 99, 341–357.

    Google Scholar 

  5. Kelly, R. B., and Rice, R. V. (1967) Science, 155, 208–210.

    Article  PubMed  CAS  Google Scholar 

  6. Kahler, G. A., Fisher, F. M., and Sass, R. L. (1976) Biol. Bull., 151, 161–181.

    Article  PubMed  CAS  Google Scholar 

  7. Kikuchi, Y., and Tamiya, N. (1987) Biochem. J., 242, 505–510.

    PubMed  CAS  Google Scholar 

  8. Marsh, M., Hamilton, G., and Sass, R. (1978) Calcif. Tiss. Res., 25, 45–51.

    Article  CAS  Google Scholar 

  9. Kikuchi, Y., Tsuchikura, O., Hirama, M., and Tamiya, N. (1987) Eur. J. Biochem., 164, 397–402.

    Article  PubMed  CAS  Google Scholar 

  10. Cao, Q., Wang, Y., and Bayley, H. (1997) Curr. Biol., 7, 677–678.

    Article  Google Scholar 

  11. Bochicchio, B., Pepe, A., and Tamburro, A. M. (2005) Chirality, 17, 346–372.

    Article  Google Scholar 

  12. Bochicchio, B., Oronoz, F. J., Pepe, A., Blanco, M., Sandberg, L. B., and Tamburro, A. M. (2005) Macromol. Biosci., 5, 502–511.

    Article  PubMed  CAS  Google Scholar 

  13. Zengqiong Huang and Gangsheng Zhang (2011) Micron, 42, 706–711.

    Article  CAS  Google Scholar 

  14. Aluigi, A., Zoccola, M., Vineis, C., Tonin, C., Ferrero, F., and Canetti, M. (2007) Int. J. Biol. Macromol., 41, 266–273.

    Article  PubMed  CAS  Google Scholar 

  15. Mecham, R. P. (2008) Methods, 45, 32–41.

    Article  PubMed  CAS  Google Scholar 

  16. Park, K. J., Jin, H. H., and Hyun, C. K. (2002) Process. Biochem., 38, 411–418.

    Article  CAS  Google Scholar 

  17. Tatham, A. S., and Shewry, P. R. (2003) in Elastomeric Proteins: Structures, Biomechanical Properties, and Biological Roles (Shewry, P. R., Tatham, A. S., and Bailey, A., eds.) Cambridge University Press, New York, pp. 338–340.

    Chapter  Google Scholar 

  18. Cardamone, J. M. (2010) J. Mol. Struct., 969, 97–105.

    Article  CAS  Google Scholar 

  19. http://www.ncbi.nlm.nih.gov/protein/NP-006112.3.

  20. Trueman, E. R. (1953) Q. J. Micro. Sci., 94, 193–202.

    Google Scholar 

  21. Morris, R. L., Hoffman, M. P., Obar, R. A., McCafferty, S. S., Gibbons, I. R., Leone, A. D., Cool, J., Allgood, E. L., Musante, A. M., Judkins, K. M., Rossetti, B. J., Rawson, A. P., and Burgess, D. R. (2006) Dev. Biol., 300, 219–237.

    Article  PubMed  CAS  Google Scholar 

  22. Trueman, E. R. (1949) Proc. Zool. Soc. Lond., 114, 717–742.

    Google Scholar 

  23. Trueman, E. R. (1951) Quart. J. Micro. Sci., 92, 129–140.

    Google Scholar 

  24. Trueman, E. R. (1954) J. Mollusc. Stud., 30, 160–166.

    Google Scholar 

  25. Dong, A., Huang, P., and Caughey, W. S. (1992) Biochemistry, 31, 182–189.

    Article  PubMed  CAS  Google Scholar 

  26. Movasaghi, Z., Rehman, S., and Rehman, I. (2008) Appl. Spectrosc. Rev., 43, 134–179.

    Article  CAS  Google Scholar 

  27. Pelton, J. T., and McLean, L. R. (2000) Anal. Biochem., 277, 167–176.

    Article  PubMed  CAS  Google Scholar 

  28. Romer, L., and Scheibel, T. (2008) in Molecular Biology Intelligence Unit: Fibrous Proteins (Scheibel, T., ed.) Landes Bioscience Press, Texas, pp. 121–127.

    Google Scholar 

  29. Sundaram, S., Deshmukh, V. D., and Raje, S. G. (2010) J. Mar. Biol. Ass. India, 52, 99–101.

    Google Scholar 

  30. Meyers, M. A., Chen, P. Y., and Lin, A. Y. M. (2008) Prog. Mater. Sci., 53, 113.

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Materials Science and Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China

    Zengqiong Huang & Gangsheng Zhang

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  1. Zengqiong Huang
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  2. Gangsheng Zhang
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Correspondence to Gangsheng Zhang.

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Published in Russian in Biokhimiya, 2011, Vol. 76, No. 11, pp. 1525–1531.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM11-155, September 11, 2011.

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Huang, Z., Zhang, G. Identification and secondary structure analysis of a keratin-like fibrous protein discovered in ligament of the bivalve Siliqua radiata . Biochemistry Moscow 76, 1227–1232 (2011). https://doi.org/10.1134/S0006297911110046

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  • DOI: https://doi.org/10.1134/S0006297911110046

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