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Effect of cholinergic drugs on the activity of basic carboxypeptidases in rat nervous tissue

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Abstract

Effects of a single administration of cholinergic drugs (arecoline, atropine, nicotine, mecamylamine) on the activity of carboxypeptidase H and of phenylmethylsulfonyl fluoride-inhibited carboxypeptidase, which are involved in metabolism of neuropeptides, were studied in brain parts and the adrenal glands of rats. The enzyme activities were determined fluorimetrically using specific inhibitors and substrates. In the majority of cases the enzyme activities decreased, and this decrease was retained for at least 72 h. Changes in the activities of the studied enzymes depended on the type of cholinergic action, the nervous system part, and the time after the injection. The changes in activities of the studied carboxypeptidases are supposed to be a possible mechanism responsible for changes in the levels of neuropeptides under the influence of high doses of the drugs.

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Abbreviations

CPH:

carboxypeptidase H

FSH:

follicle-stimulating hormone

GABA:

γ-aminobutyric acid

GEMSA:

guanidine ethylmercaptosuccinic acid

GnRF:

gonadotropin-releasing factor

LH:

luteinizing hormone

PMSF-CP:

phenylmethylsulfonyl fluoride-inhibited carboxypeptidase

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Authors and Affiliations

  1. Department of Biochemistry, Belinsky Penza State Pedagogical University, ul. Lermontova 37, 440026, Penza, Russia

    V. B. Solov’ev, M. T. Gengin, O. V. Solov’eva, O. V. Pavlova & E. S. Lobzina

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  1. V. B. Solov’ev
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  2. M. T. Gengin
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  3. O. V. Solov’eva
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  4. O. V. Pavlova
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  5. E. S. Lobzina
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Correspondence to V. B. Solov’ev.

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Original Russian Text © V. B. Solov’ev, M. T. Gengin, O. V. Solov’eva, O. V. Pavlova, E. S. Lobzina, 2011, published in Biokhimiya, 2011, Vol. 76, No. 10, pp. 1437–1443.

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Solov’ev, V.B., Gengin, M.T., Solov’eva, O.V. et al. Effect of cholinergic drugs on the activity of basic carboxypeptidases in rat nervous tissue. Biochemistry Moscow 76, 1172–1177 (2011). https://doi.org/10.1134/S0006297911100117

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  • DOI: https://doi.org/10.1134/S0006297911100117

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