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Interaction of transketolase from human tissues with substrates

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Abstract

The Michaelis constant values for substrates of transketolase from human tissues were determined over a wide range of substrate concentrations. It is shown that K m values determined by other authors are significantly overestimated and explained why this is so.

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Abbreviations

DOETDP:

α,β-dioxyethylthiamine diphosphate

DTT:

dithiothreitol

F-6-P:

fructose-6-phosphate

GAPD:

glyceraldehyde-3-phosphate dehydrogenase

hTK:

human transketolase

R-5-P:

ribose-5-phosphate

sTK:

Saccharomyces cerevisiae transketolase

TDP:

thiamine diphosphate

TK:

transketolase

X-5-P:

xylulose-5-phosphate

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    L. E. Meshalkina, O. N. Solovjeva & G. A. Kochetov

Authors
  1. L. E. Meshalkina
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  2. O. N. Solovjeva
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  3. G. A. Kochetov
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Correspondence to L. E. Meshalkina.

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Original Russian Text © L. E. Meshalkina, O. N. Solovjeva, G. A. Kochetov, 2011, published in Biokhimiya, 2011, Vol. 76, No. 9, pp. 1300–1304.

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Meshalkina, L.E., Solovjeva, O.N. & Kochetov, G.A. Interaction of transketolase from human tissues with substrates. Biochemistry Moscow 76, 1061–1064 (2011). https://doi.org/10.1134/S0006297911090112

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  • DOI: https://doi.org/10.1134/S0006297911090112

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