Abstract
Placenta is a source of carbohydrate-binding proteins that function as molecular scavengers, but they could also be involved in interactions that assist in metabolic control. Mannose/N-acetyl-glucosamine (Man/GlcNAc)-binding proteins from placenta were isolated and their reactivity towards placental insulin and insulin-like growth factor receptors (IR and IGF-Rs) was analyzed. The lectins reduced the binding of insulin and IGF-I in a dose-dependent manner, while almost no effect was observed on the binding of IGF-II. The shape of the inhibition curves changed, suggesting altered binding specificity. The presence of sugar could not reverse completely the effect of the lectins, implicating both lectin-sugar and protein-protein conformational recognition. Since biological molecules in our experimental system were those that are in close relation in vivo, placental Man/GlcNAc-specific lectins may be regarded as potential allosteric modulators of lig- and-receptor interactions in a system of homologous ligands, selectively affecting only binding to tyrosine kinase type receptors (IR and IGF-1R).
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Abbreviations
- CIM6P/IGF-II-R:
-
cation-independent mannose-6-phosphate/IGF-II receptor
- Con A:
-
concanavalin A
- GlcNAc:
-
N-acetyl-glucosamine
- HbA1c:
-
glycosylated (glycated) hemoglobin
- HBS:
-
Hepes buffered saline
- IDDM:
-
insulin-dependent diabetes mellitus
- IGF:
-
insulin-like growth factor
- IR:
-
insulin receptor
- IGF-1R:
-
type 1 IGF receptor
- IGF-2R:
-
type 2 IGF receptor
- Man:
-
mannose
- MBL:
-
mannose-binding lectin
- MMR:
-
macrophage mannose receptor
- PBS:
-
phosphate buffered saline
- WGA:
-
wheat germ agglutinin
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Published in Russian in Biokhimiya, 2011, Vol. 76, No. 9, pp. 1230–1237.
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Nedić, O., Filimonović, D., Miković, Z. et al. Influence of placental mannose/N-acetyl glucosamine-binding proteins on the interaction of insulin and insulin-like growth factors with their receptors. Biochemistry Moscow 76, 1003–1008 (2011). https://doi.org/10.1134/S0006297911090033
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DOI: https://doi.org/10.1134/S0006297911090033