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Galectins promote the interaction of influenza virus with its target cell

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Abstract

Influenza virus is known to bind sialoglycans located on the surface of the host cell. In addition, recent data suggest the involvement of other molecular targets in viral reception. Of note, a high density of terminal galactose residues is created on the surface of virions because of the influenza virus’ own neuraminidase activity. Thus, we suggested the possibility for an interaction of the influenza virus with galactose-binding proteins — galectins. In the present work we studied the influence of several galectins on the adhesion and further internalization of virus into the cell; six virus strains and three cell lines were studied. Chicken galectins CG-1A and -2 as well as human galectins HGal-1 and -8 promote virus binding in dose dependent manner, but they do not influence the internalization stage. Also, galectins are able to restore the ability of influenza virus to infect desialylated cells up to the level of native cells. When CG-1A in physiological concentrations was loaded onto viruses, the adhesion level was higher than in the case of on-cell loading. The effect of adhesion increase depends on the glycan structure of target-cell as well as of virus. The aggregated data suggest a promotional effect of galectins during the stage of influenza virus binding with the surface of target-cell.

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Abbreviations

BSA:

bovine serum albumin

C-virus:

virus passaged in chicken embryos

CG:

chicken galectins

FITC:

fluorescein isothiocyanate

HA:

hemagglutinin

HGal:

human galectins

M-virus:

virus passaged in MDCK (Madin-Darby Canine Kidney) cells

NA:

neuraminidase

Neu5Ac:

N-acetyl-neuraminic acid

PBA:

phosphate buffered saline containing 0.2% BSA

PBS:

phosphate buffered saline

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    E. S. Chernyy, E. M. Rapoport & N. V. Bovin

  2. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Veterinar str. 13, 80539, Munich, Germany

    S. Andre, H. Kaltner & H. -J. Gabius

Authors
  1. E. S. Chernyy
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  2. E. M. Rapoport
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  3. S. Andre
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  4. H. Kaltner
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  5. H. -J. Gabius
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  6. N. V. Bovin
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Correspondence to N. V. Bovin.

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Published in Russian in Biokhimiya, 2011, Vol. 76, No. 8, pp. 1173–1184.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM11-041, April 24, 2011.

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Chernyy, E.S., Rapoport, E.M., Andre, S. et al. Galectins promote the interaction of influenza virus with its target cell. Biochemistry Moscow 76, 958–967 (2011). https://doi.org/10.1134/S0006297911080128

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