Abstract
Partially purified endopolyphosphatase from cytosol of the yeast Saccharomyces cerevisiae with inactivated genes PPX1 and PPN1 encoding exopolyphosphatases was obtained with ion_exchange and affinity chromatography. The enzyme activity was estimated by decrease of polyphosphate chain length determined by PAGE. The enzyme cleaved inorganic polyphosphate without the release of orthophosphate (Pi) and was inhibited by heparin and insensitive to fluoride. Mg2+, Mn2+, and Co2+ (1.5 mM) stimulated the activity, and Ca2+ was ineffective. The molecular mass of the endopolyphosphatase determined by gel filtration was of ≈20 kDa.
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Published in Russian in Biokhimiya, 2010, Vol. 75, No. 11, pp. 1596–1600.
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Lichko, L.P., Kulakovskaya, T.V. & Kulaev, I.S. Properties of Partially Purified Endopolyphosphatase of the Yeast Saccharomyces cerevisiae . Biochemistry Moscow 75, 1404–1407 (2010). https://doi.org/10.1134/S0006297910110131
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DOI: https://doi.org/10.1134/S0006297910110131