Skip to main content
SpringerLink
Log in

Influence of Distamycin, Chromomycin, and UV-irradiation on Extraction of Histone H1 from Rat Liver Nuclei by Polyglutamic Acid

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

Rat liver nucleus histone H1 was fractionated by polyglutamic acid (PG) in the presence of distamycin A (DM) or chromomycin A3 (CM). In the absence of the antibiotics, PG extracts from the nuclei about half of the nuclear H1. DM or CM added to the nuclei in saturating concentrations weakens the binding potential of most of H1. Titration of nuclei with DM shows that the number of binding sites for DM in the nuclei is less than in isolated DNA by only 20-25%, and this dif- ference disappears after treatment of nuclei with PG. The lower CD value of DM complexes with nuclei compared to that of DM complexes with free DNA is evidence of a change in the DM-DNA binding mode in nuclear chromatin. About 25% of total histone H1 is sensitive only to DM and s~5% is sensitive only to CM. Half of the DM-sensitive H1 fraction seems to have a different binding mode in condensed compared relaxed chromatin. A small part of H1 (s~3%) remains tightly bound to the nuclear chromatin independent of the presence of the antibiotics. Subfraction H1A is more DM-sensitive and H1B is more CM-sensitive. UV irradiation of nuclei results in dose-dependent cross-linking of up to 50% of total H1, which is neither acid-extractable nor recovered during SDS electrophoresis. PG with DM extracts only about 3% of H1 from UV- stabilized chromatin. DM treatment of the nuclei before UV irradiation results in extraction of the whole DM-sensitive H1 fraction (s~25%), which in this case is not stabilized in the nucleus. A hypothesis on possible roles of the found H1 fractions in chromatin structural organization is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zlatanova, J., and van Holde, K. (1996) Prog. Nuceic. Acids Res. Mol. Biol., 52, 217–259.

    Article  CAS  Google Scholar 

  2. Yao, J., Lowary, P. T., and Widom, J. (1991) Biochemistry, 30, 8408–8414.

    Article  CAS  PubMed  Google Scholar 

  3. Nacheva, G. A., Guschin, D. Y., Preobrazhenskaya, O. V., Karpov, V. L., Ebralidse, K. K., and Mirzabekov, A. D. (1989) Cell, 58, 27–36.

    Article  CAS  PubMed  Google Scholar 

  4. Pruss, D., Bartholomew, B., Persinger, J., Hayes, J., Arents, G., Moudrianakis, E. N., and Wolffe, A. P. (1996) Science, 25, 614–617.

    Article  Google Scholar 

  5. Nelson, P. P., Albright, S. C., Wiseman, J. M., and Garrard, W. T. (1979) J. Biol. Chem., 254, 11751–11760.

    CAS  PubMed  Google Scholar 

  6. Ponte, I., Vidal-Taboada, J. M., and Suau, P. (1998) Mol. Biol. Evol., 15, 702–708.

    CAS  PubMed  Google Scholar 

  7. Liao, L. W., and Cole, R. D. (1981) J. Biol. Chem., 256, 10124–10128.

    CAS  PubMed  Google Scholar 

  8. Khadake, J. R., and Rao, M. R. S. (1995) Biochemistry, 34, 15792–15801.

    Article  CAS  PubMed  Google Scholar 

  9. Th’ng, J. P., Sung, R., Ye, M., and Hendzel, M. J. (2005) J. Biol. Chem., 280, 27809–27814.

    Article  PubMed  Google Scholar 

  10. Catez, F., Ueda, T., and Bustin, M. (2006) Nat. Struct. Mol. Biol., 13, 305–310.

    Article  CAS  PubMed  Google Scholar 

  11. Renz, M., and Day, L. A. (1976) Biochemistry, 15, 3220–3228.

    Article  CAS  PubMed  Google Scholar 

  12. Caiafa, P., Reale, A., D’Erme, M., Allegra, P., Santoro, R., and Strom, R. (1991) Biochim. Biophys. Acta, 1129, 43–48.

    CAS  PubMed  Google Scholar 

  13. Kas, E., Poljak, L., Adachi, Y., and Laemmli, U. K. (1993) EMBO J., 12, 115–126.

    CAS  PubMed  Google Scholar 

  14. Gasser, S. M., Laroche, T., Falquet, J., Boy de la Tour, E., and Laemmli, U. K. (1986) J. Mol. Biol., 188, 613–629.

    Article  CAS  PubMed  Google Scholar 

  15. Spirin, A. S. (1958) Biokhimiya, 23, 656–661.

    CAS  Google Scholar 

  16. Laemmli, U. K. (1970) Nature, 227, 680–685.

    Article  CAS  PubMed  Google Scholar 

  17. Villeponteau, B. (1992) Biochem. J., 288, 953–958.

    CAS  PubMed  Google Scholar 

  18. Luck, G., Zimmer, C., Reinert, K. E., and Arcamone, F. (1977) Nucleic Acids Res., 4, 2655–2670.

    Article  CAS  PubMed  Google Scholar 

  19. Schultz, P. G., and Dervan, P. B. (1984) J. Biomol. Struct. Dyn., 1, 1133–1147.

    CAS  PubMed  Google Scholar 

  20. Melnikova, A. F., Zasedatelev, A. S., Kolchinsky, A. M., Gursky, G. V., Zhuze, A. L., Grochovsky, S. L., and Mirzabekov, A. D. (1975) Mol. Biol. Rep., 2, 135–142.

    Article  CAS  PubMed  Google Scholar 

  21. Fodera, R., Caneva, R., Canzonetta, C., and Savino, M. (2000) Nucleosides Nucleotides Nucleic Acids, 19, 1231–1240.

    Article  CAS  PubMed  Google Scholar 

  22. Krylov, A. S., Grokhovsky, S. L., Zasedatelev, A. S., Zhuze, A. L., Gursky, G. V., and Gottikh, B. P. (1979) Nucleic Acids Res., 6, 289–304.

    Article  CAS  PubMed  Google Scholar 

  23. Bhattacharya, S., and Thomas, M. (2000) Biochem. Biophys. Res. Commun., 267, 139–144.

    Article  CAS  PubMed  Google Scholar 

  24. Skamrov, A. V., Rybalkin, I. N., Bibilashvili, R. Sh., Gottikh, B. P., and Grokholsky, S. L. (1985) Mol. Biol. (Moscow), 19, 177–195.

    CAS  Google Scholar 

  25. Mir, M. A., Majee, S., Das, S., and Dasgupta, D. (2003) Bioorg. Med. Chem., 11, 2791–2801.

    Article  CAS  PubMed  Google Scholar 

  26. Behr, W., Wonipel, K., and Hartmann, G. (1969) Eur. J. Biochem., 9, 82–92.

    Article  CAS  PubMed  Google Scholar 

  27. Kharchenko, E. P., Miul’berg, A. A., Tishchenko, L. D., and Borisova, G. A. (1982) Biokhimiya, 47, 713–723.

    CAS  Google Scholar 

  28. Marx, K. A., Zhou, Y., and Kishawi, I. Q. (2006) J. Biomol. Struct. Dyn., 23, 429–446.

    CAS  PubMed  Google Scholar 

  29. Wellman, S. E., Sittman, D. B., and Chaires, J. B. (1994) Biochemistry, 33, 384–388.

    Article  CAS  PubMed  Google Scholar 

  30. Wellman, S. E., Song, Y., and Mamoon, N. M. (1999) Biochemistry, 38, 13112–13118.

    Article  CAS  PubMed  Google Scholar 

  31. Happel, N., and Doenecke, D. (2009) Gene, 431, 1–12.

    Article  CAS  PubMed  Google Scholar 

  32. Lindner, H., Helliger, W., and Puschendorf, B. (1990) Biochem. J., 269, 359–363.

    CAS  PubMed  Google Scholar 

  33. Breneman, J. W., Yau, P., Teplitz, R. L., and Bradbury, E. M. (1993) Exp. Cell Res., 206, 16–26.

    Article  CAS  PubMed  Google Scholar 

  34. Banchev, T., Srebreva, L., Zlatanova, J., and Tsanev, R. (1988) Exp. Cell Res., 177, 1–8.

    Article  CAS  PubMed  Google Scholar 

  35. Orrego, M., Ponte, I., Roque, A., Buschati, N., Mora, X., and Suau, P. (2007) BMC Biol., 5, 22.

    Article  PubMed  Google Scholar 

  36. Bianchi, N. O., Morgan, W. F., and Cleaver, J. E. (1985) Exp. Cell Res., 156, 405–418.

    Article  CAS  PubMed  Google Scholar 

  37. Martinson, H. G., Shetlar, M. D., and McCarthy, B. J. (1976) Biochemistry, 15, 2002–2007.

    Article  CAS  PubMed  Google Scholar 

  38. Cao, M., and Sung, M. (1982) Biochemistry, 21, 3419–3427.

    Article  CAS  PubMed  Google Scholar 

  39. Fais, D., Prusov, A. N., and Polyakov, V. Yu. (1989) Cell Biol. Int. Rep., 13, 747–758.

    Article  CAS  PubMed  Google Scholar 

  40. Celis, J. E., Fink, M., and Kaltoft, K. (1976) Nucleic Acids Res., 3, 1065–1071.

    CAS  PubMed  Google Scholar 

  41. Prusov, A. N., and Kolomijtseva, G. Ya. (1997) Biochemistry (Moscow), 62, 665–672.

    Google Scholar 

  42. Lalwani, R., Maiti, S., and Mukherji, S. (1995) J. Photochem. Photobiol., 27, 117–122.

    Article  CAS  Google Scholar 

  43. Prusov, A. N., Kireev, I. I., and Polyakov, V. Y. (2003) Photochem. Photobiol., 78, 592–598.

    Article  CAS  PubMed  Google Scholar 

  44. Van Holde, K., and Zlatanova, J. (2007) Semin. Cell Dev. Biol., 18, 651–658.

    Article  PubMed  Google Scholar 

  45. Klingholz, R., Stratling, W. H., and Schafer, H. (1981) Exp. Cell Res., 132, 399–409.

    Article  CAS  PubMed  Google Scholar 

  46. Prusov, A. N., Polyakov, V. Yu., Zatsepina, O. V., Chentsov, Yu. S., and Fais, D. (1983) Cell Biol. Int. Rep., 7, 849–858.

    Article  CAS  PubMed  Google Scholar 

  47. Kiryanov, G. I., Manamshjan, T. A., Polyakov, V. Y., Fais, D., and Chentsov, J. S. (1976) FEBS Lett., 67, 323–327.

    Article  CAS  PubMed  Google Scholar 

  48. Hozier, J., Renz, M., and Nehls, P. (1977) Chromosoma, 62, 301–317.

    Article  CAS  PubMed  Google Scholar 

  49. Stratling, W. H., Muller, U., and Zentgraf, H. (1978) Cell Biol. Int. Rep., 2, 495–499.

    Article  CAS  PubMed  Google Scholar 

  50. Kiryanov, G. I., Smirnova, T. A., and Polyakov, V. Yu. (1982) Eur. J. Biochem., 124, 331–338.

    Article  CAS  PubMed  Google Scholar 

  51. Burgoyne, L. A., and Skinner, J. D. (1981) Biochem. Biophys. Res. Commun., 99, 893–899.

    Article  CAS  PubMed  Google Scholar 

  52. Pospelov, V. A., and Svetlikova, S. B. (1982) Mol. Biol. (Moscow), 16, 1034–1040.

    CAS  Google Scholar 

  53. Kiryanov, G. I., Smirnova, T. A., Manamshyan, T. A., and Khodosovskaya, A. M. (1987) Biokhimiya, 52, 1855–1866.

    CAS  Google Scholar 

  54. Renz, M. (1975) Proc. Natl. Acad. Sci. USA, 72, 733–736.

    Article  CAS  PubMed  Google Scholar 

  55. Suciu, D., and Uray, Z. (1980) Acta Biol. Med. Ger., 39, 1101–1109.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    A. N. Prusov, T. A. Smirnova, L. P. Kurochkina & G. Ya. Kolomijtseva

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    L. P. Kurochkina

Authors
  1. A. N. Prusov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. A. Smirnova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. P. Kurochkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Ya. Kolomijtseva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. N. Prusov.

Additional information

Original Russian Text © A. N. Prusov, T. A. Smirnova, L. P. Kurochkina, G. Ya. Kolomijtseva, 2010, published in Biokhimiya, 2010, Vol. 75, No. 11, pp. 1508–1521.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prusov, A.N., Smirnova, T.A., Kurochkina, L.P. et al. Influence of Distamycin, Chromomycin, and UV-irradiation on Extraction of Histone H1 from Rat Liver Nuclei by Polyglutamic Acid. Biochemistry Moscow 75, 1331–1341 (2010). https://doi.org/10.1134/S0006297910110040

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297910110040

Key words

Search

Navigation