Abstract
The structure of the oligomeric protein α-crystallin from bovine eye lens was investigated by small-angle neutron scattering (SANS) with contrast variation. Based on the SANS curves, the match point for α-crystallin (43% D2O) and its average scattering length density at this point (2.4•1010 cm-2) were evaluated. The radius of gyration and the distance distri- bution functions for α-crystallin were calculated. On the basis of these calculations, it was concluded that α-crystallin is characterized by homogeneous distribution of scattering density in the domains inaccessible for water penetration, and all polypeptide subunits in α-crystallin oligomers undergo equal deuteration. The latter indicates that all α-crystallin subunits are equally accessible for water and presumably for some other low molecular weight substances. These conclusions on the α-crystallin structure (homogeneous distribution of scattering density and equal accessibility of all subunits for low molecular weight substances) should be taken into account when elaborating a-crystallin quaternary structure models.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Horwitz, J. (2003) Exp. Eye Res., 76, 145–153.
Tardieu, A., Laporte, D., Lichino, P., Krop, B., and Delae, M. (1986) J. Mol. Biol., 192, 711–724.
Krivandin, A. V., Muranov, K. O., Yakovlev, F. Yu., Poliansky, N. B., Wasserman, L. A., and Ostrovsky, M. A. (2009) Biochemistry (Moscow), 74, 633–642.
Horwitz, J. (1992) Proc. Natl. Acad. Sci. USA, 89, 10449–10453.
Krivandin, A. V., Muranov, K. O., and Ostrovsky, M. A. (2004) Mol. Biol. (Moscow), 38, 532–546.
Krivandin, A. V., Muranov, K. O., Poturaeva, I. D., Poliansky, N. B., and Ostrovsky, M. A. (2006) Dokl. AN, 409, 550–554.
Groth-Vasselli, B., Kumosinski, T. F., and Farnsworth, P. N. (1995) Exp. Eye Res., 61, 249–253.
Peschek, J., Braun, N., Franzmann, T. M., Georgalis, Y., Haslbeck, M., Weinkauf, S., and Buchner, J. (2009) Proc. Natl. Acad. Sci. USA, 106, 13272–13277.
Feigin, L. A., and Svergun, D. I. (1987) Structure Analysis by Small-Angle X-Ray and Neutron Scattering, Plenum Press, New York.
Ostanevich, Yu. M., and Serdyuk, I. I. (1982) Uspekhi Fiz. Nauk, 137, 85–11.
Kuklin, A. I., Islamov, A. Kh., and Gordeliy, V. I. (2005) Neutron News, 16, 16–18.
Solovyev, A. G., Solovyeva, T. M., Stadnik, A. V., Islamov, A. Kh., and Kuklin, A. I. (2003) JINR Communication P10-2003-86, JINR, Dubna.
Svergun, D. I., and Semenyuk, A. V. (1987) Dokl. AN SSSR, 297, 1373–1377.
Svergun, D. I. (1992) J. Appl. Cryst., 25, 495–503.
Avdeev, M. V. (2007) J. Appl. Cryst., 40, 56–70.
Stuhrmann, H. B., and Miller, A. (1978) J. Appl. Cryst., 11, 325–345.
Haley, D. A., Horwitz, J., and Stewart, P. L. (1998) J. Mol. Biol., 277, 27–35.
Svergun, D. I., Richard, S., Koch, M. H. J., Sayers, Z., Kuprin, S., and Zaccai, G. (1998) Proc. Natl. Acad. Sci. USA, 95, 2267–2272.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A. V. Krivandin, T. N. Murugova, A. I. Kuklin, K. O. Muranov, N. B. Poliansky, V. L. Aksenov, M. A. Ostrovsky, 2010, published in Biokhimiya, 2010, Vol. 75, No. 11, pp. 1499–1507.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM10-151, October 10, 2010.
Rights and permissions
About this article
Cite this article
Krivandin, A.V., Murugova, T.N., Kuklin, A.I. et al. Study of α-Crystallin Structure by Small Angle Neutron Scattering with Contrast Variation. Biochemistry Moscow 75, 1324–1330 (2010). https://doi.org/10.1134/S0006297910110039
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297910110039