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Inorganic polyphosphates in mitochondria

Biochemistry (Moscow) volume 75, pages 825–831 (2010)Cite this article

Abstract

Current data concerning the crucial role of inorganic polyphosphates (polyP) in mitochondrial functions and dysfunctions in yeast and animal cells are reviewed. Biopolymers with short chain length (∼15 phosphate residues) were found in the mitochondria of Saccharomyces cerevisiae. They comprised 7–10% of the total polyP content of the cell. The polyP are located in the membranes and intermembrane space of mitochondria. The mitochondrial membranes possess polyP/Ca2+/polyhydroxybutyrate complexes. PolyP accumulation is typical of promitochondria but not of functionally active mitochondria. Yeast mitochondria possess two exopolyphosphatases splitting Pi from the end of the polyP chain. One of them, encoded by the PPX1 gene, is located in the matrix; the other one, encoded by the PPN1 gene, is membrane-bound. Formation of well-developed mitochondria in the cells of S. cerevisiae after glucose depletion is accompanied by decrease in the polyP level and the chain length. In PPN1 mutants, the polyP chain length increased under glucose consumption, and the formation of well-developed mitochondria was blocked. These mutants were defective in respiration functions and consumption of oxidizable carbon sources such as lactate and ethanol. Since polyP is a compound with high-energy bonds, its metabolism vitally depends on the cell bioenergetics. The maximal level of short-chain acid-soluble polyP was observed in S. cerevisiae under consumption of glucose, while the long-chain polyP prevailed under ethanol consumption. In insects, polyP in the mitochondria change drastically during ontogenetic development, indicating involvement of the polymers in the regulation of mitochondrial metabolism during ontogenesis. In human cell lines, specific reduction of mitochondrial polyP under expression of yeast exopolyphosphatase PPX1 significantly modulates mitochondrial bioenergetics and transport.

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Abbreviations

PHB:

polyhydroxybutyrate

polyP:

inorganic polyphosphates

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Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290, Pushchino, Moscow Region, Russia

    T. V. Kulakovskaya, L. P. Lichko, V. M. Vagabov & I. S. Kulaev

Authors
  1. T. V. Kulakovskaya
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  2. L. P. Lichko
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  3. V. M. Vagabov
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  4. I. S. Kulaev
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Corresponding author

Correspondence to T. V. Kulakovskaya.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 7, pp. 936–943.

On March 26, 2010 Igor Stepanovich Kulaev celebrated his 80th anniversary. The coauthors congratulate him with warm wishes of health and success in science.

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Kulakovskaya, T.V., Lichko, L.P., Vagabov, V.M. et al. Inorganic polyphosphates in mitochondria. Biochemistry Moscow 75, 825–831 (2010). https://doi.org/10.1134/S0006297910070035

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  • DOI: https://doi.org/10.1134/S0006297910070035

Key words

  • inorganic polyphosphate
  • mitochondria
  • polyP/Ca2+/polyhydroxybutyrate
  • exopolyphosphatase
  • membrane potential
  • glucose repression
  • yeast
  • animal
  • insect