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Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form)

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Abstract

An acidic O-specific polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-galactose, 2,6-dideoxy-2-(N-acetyl-L-threonine)amino-D-galactose, and 2-acetamido-2-deoxy-D-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form) followed by gel-permeation chromatography. The polysaccharide was subjected to Smith degradation to give a modified polysaccharide with trisaccharide repeating unit containing L-threonine. The initial and modified polysaccharides were studied by sugar analysis and 1H- and 13C-NMR spectroscopy, including COSY, TOCSY, ROESY, and HSQC experiments, and the structure of the branched tetrasaccharide repeating unit of the polysaccharide was established.

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Abbreviations

COSY:

correlation spectroscopy

HSQC:

het-eronuclear single-quantum coherence

ROESY:

rotating-frame nuclear Overhauser effect spectroscopy

TOCSY:

total correlation spectroscopy

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, 690022, Vladivostok, Russia

    N. A. Komandrova, V. V. Isakov, S. V. Tomshich & L. A. Romanenko

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia

    A. V. Perepelov & A. S. Shashkov

Authors
  1. N. A. Komandrova
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  2. V. V. Isakov
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  3. S. V. Tomshich
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  4. L. A. Romanenko
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  5. A. V. Perepelov
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  6. A. S. Shashkov
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Corresponding author

Correspondence to N. A. Komandrova.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 5, pp. 727–734.

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Komandrova, N.A., Isakov, V.V., Tomshich, S.V. et al. Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form). Biochemistry Moscow 75, 623–628 (2010). https://doi.org/10.1134/S0006297910050123

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  • DOI: https://doi.org/10.1134/S0006297910050123

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