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Proteomic analysis of salicylate-induced proteins of pea (Pisum sativum L.) leaves

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Abstract

The effect of 50 μM salicylic acid on soluble proteins of pea (Pisum sativum L.) leaves was studied by proteomic analysis. Thirty-two salicylate-induced proteins were found, and 13 of these were identified using MALDI TOF MS. Salicylate-induced increased content was shown for the first time for the family 18 glycoside hydrolase, α-amylase, 33 kDa protein of photosystem II, lipid-desaturase-like protein, and glutamine amidotransferase. Increased content of protective proteins of direct antipathogenic action such as chitinase and β-1,3-glucanases was also noted.

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Abbreviations

Bio-Lyte (pH 3-10):

ampholytes

Chaps:

3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (nonionic detergent)

2-D electrophoresis:

two-dimensional electrophoresis

IEF:

isoelectric focusing

NPR1:

protein that does not cause expression of the pathogen-induced protein PR1 (non-expressor of PR1)

SA:

salicylic acid

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, ul. Lobachevskogo 2/31, 420111, Kazan, Russia

    I. A. Tarchevsky, V. G. Yakovleva & A. M. Egorova

Authors
  1. I. A. Tarchevsky
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  2. V. G. Yakovleva
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  3. A. M. Egorova
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Correspondence to I. A. Tarchevsky.

Additional information

Original Russian Text © I. A. Tarchevsky, V. G. Yakovleva, A. M. Egorova, 2010, published in Biokhimiya, 2010, Vol. 75, No. 5, pp. 689–697.

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Tarchevsky, I.A., Yakovleva, V.G. & Egorova, A.M. Proteomic analysis of salicylate-induced proteins of pea (Pisum sativum L.) leaves. Biochemistry Moscow 75, 590–597 (2010). https://doi.org/10.1134/S0006297910050081

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  • DOI: https://doi.org/10.1134/S0006297910050081

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