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Heterogeneous set of cell wall teichoic acids in strains of Bacillus subtilis VKM B-760 and VKM B-764

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Abstract

Cell walls of Bacillus subtilis VKM B-760 and VKM B-764 are characterized by heterogeneous composition of teichoic acids. Polymer I with structure -6)-β-D-Galp-(1→1)-sn-Gro-(3-P-, polymer II with structure -6)-α-D-Glcp-(1→1)-sn-Gro-(3-P-, and a small amount of unsubstituted 1,3-poly(glycerol phosphate) were detected in strain VKM B-760. Strain VKM B-764 contains an analogous set of teichoic acids, but a characteristic feature of polymer II is the presence of disubstituted glycerol residue with α-glucopyranose localization in the integral chain at C-1 hydroxyl and β-glucopyranose as a side branch at C-2 hydroxyl (polymer III): -6)-α-D-Glcp-(1→1)-[β-D-Glcp-(1→2)]-sn-Gro-(3-P-. The structures of polymer I in bacilli and polymer III in Gram-positive bacteria are described for the first time. Teichoic acids were studied by chemical methods and on the basis of combined analysis of one-dimensional 1H-, 13C-, and 31P-NMR spectra, homonuclear two-dimensional 1H/1H COSY, TOCSY, and ROESY, and heteronuclear two-dimensional 1H/13C gHSQC- and HMQC-TOCSY experiments. Simultaneous presence of several different structure teichoic acids in the bacillus cell walls as well as chemotaxonomical perspectives of the application of these polymers as species-specific markers for members of the Bacillus genus is discussed.

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Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119899, Moscow, Russia

    N. V. Potekhina, G. M. Streshinskaya & Yu. I. Kozlova

  2. All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142292, Pushchino, Moscow Region, Russia

    E. B. Kudryashova

  3. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119992, Moscow, Russia

    S. N. Senchenkova & A. S. Shashkov

  4. Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, ul. Goleva 13, 614081, Perm’, Russia

    L. N. Anan’ina

Authors
  1. N. V. Potekhina
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  2. G. M. Streshinskaya
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  3. Yu. I. Kozlova
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  4. E. B. Kudryashova
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  5. S. N. Senchenkova
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  6. A. S. Shashkov
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  7. L. N. Anan’ina
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Correspondence to N. V. Potekhina.

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Original Russian Text © N. V. Potekhina, G. M. Streshinskaya, Yu. I. Kozlova, E. B. Kudryashova, S. N. Senchenkova, A. S. Shashkov, L. N. Anan’ina, 2009, published in Biokhimiya, 2009, Vol. 74, No. 12, pp. 1678–1685.

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Potekhina, N.V., Streshinskaya, G.M., Kozlova, Y.I. et al. Heterogeneous set of cell wall teichoic acids in strains of Bacillus subtilis VKM B-760 and VKM B-764. Biochemistry Moscow 74, 1368–1374 (2009). https://doi.org/10.1134/S0006297909120116

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  • DOI: https://doi.org/10.1134/S0006297909120116

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