Abstract
A method using high resolution atomic force microscopy for imaging DNA has been elaborated. Using super-sharp probes and modified graphite as support for molecule adsorption, DNA molecule images were obtained whose resolution made possible the observation of their fine structure with repeated helical motifs. The method can be used to visualize individual spread molecules of single-stranded DNA.
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Abbreviations
- AFM:
-
atomic-force microscopy
- HOPG:
-
highly oriented pyrolitic graphite
- ssDNA:
-
single-stranded DNA
References
Uvarov, V. Yu., Ivanov, Yu. D., Romanov, A. N., Gallyamov, M. O., Kiselyova, O. I., and Yaminsky, I. V. (1996) Biochimie, 78, 780–784.
Rees, W. A., Keller, R. W., Vesenka, J. P., Yang, G., and Bustamante, C. (1993) Science, 260, 1646–1649.
Guthold, M., Bezanilla, M., Erie, D. A., Jenkins, B., Hansma, H. G., and Bustamante, C. (1994) Proc. Natl. Acad. Sci. USA, 91, 12927–12931.
Murray, N., Hansma, H. G., Bezanilla, M., Sano, T., Ogletree, D. F., Kolbe, W., Smith, C. L., Cantor, C. R., Spengler, S., Hansma, P. K., and Salmeron, M. (1993) Proc. Natl. Acad. Sci. USA, 90, 3811–3814.
Klinov, D. V. (1997) Abstract of Candidate’s Dissertation [in Russian], Moscow Physico-Technical Institute, Moscow.
Klinov, D. V., Lagutina, I. V., Prokhorov, V. V., Neretina, T. V., Khil, P. P., Lebedev, Yu. B., Cherny, D. I., Demin, V. V., and Sverdlov, E. D. (1998) Nucleic Acids Res., 26, 4603–610.
Klinov, D., and Magonov, S. (2004) Appl. Phys. Lett., 84, 2697–2699.
Adamcik, J., Klinov, D. V., Witz, G., Sekatskii, S. K., and Dietler, G. (2006) FEBS Lett., 580, 5671–5675.
Klinov, D. V., Martynkina, L. P., Yurchenko, V. Yu., Demin, V. V., Streltsov, S. A., Gerasimov, Yu. A., and Vengerov, Yu. Yu. (2003) Bioorg. Khim., 29, 363–367.
Cheung, C. L., Hafner, J. H., and Lieber, C. M. (2000) Proc. Natl. Acad. Sci. USA, 97, 3809–3813.
Mou, J., Czajkowsky, D. M., Zhang, Y., and Shao, Z. (1995) FEBS Lett., 371, 279–282.
Klinov, D., Dwir, B., Kapon, E., Borovok, N., Molotsky, T., and Kotlyar, A. (2007) Nanotechnology, 18, 202–212.
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Original Russian Text © D. V. Klinov, T. V. Neretina, V. V. Prokhorov, T. V. Dobrynina, K. G. Aldarov, V. V. Demin, 2009, published in Biokhimiya, 2009, Vol. 74, No. 10, pp. 1410–1415.
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Klinov, D.V., Neretina, T.V., Prokhorov, V.V. et al. High-resolution atomic force microscopy of DNA. Biochemistry Moscow 74, 1150–1154 (2009). https://doi.org/10.1134/S0006297909100113
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DOI: https://doi.org/10.1134/S0006297909100113