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Prediction of amino acid residues protected from hydrogen-deuterium exchange in a protein chain

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Abstract

We have investigated the possibility to predict protection of amino acid residues from hydrogen-deuterium exchange. A database containing experimental hydrogen-deuterium exchange data for 14 proteins for which these data are known has been compiled. Different structural parameters related to flexibility of amino acid residues and their amide groups have been analyzed to answer the question whether these parameters can be used for predicting the protection of amino acid residues from hydrogen-deuterium exchange. A method for prediction of protection of amino acid residues, which uses only the amino acid sequence of a protein, has been elaborated.

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Authors and Affiliations

  1. Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    N. V. Dovidchenko, M. Yu. Lobanov, S. O. Garbuzynskiy & O. V. Galzitskaya

Authors
  1. N. V. Dovidchenko
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  2. M. Yu. Lobanov
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  3. S. O. Garbuzynskiy
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  4. O. V. Galzitskaya
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Corresponding author

Correspondence to O. V. Galzitskaya.

Additional information

Published in Russian in Biokhimiya, 2009, Vol. 74, No. 8, pp. 1091–1102.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM09-044, June 28, 2009.

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Dovidchenko, N.V., Lobanov, M.Y., Garbuzynskiy, S.O. et al. Prediction of amino acid residues protected from hydrogen-deuterium exchange in a protein chain. Biochemistry Moscow 74, 888–897 (2009). https://doi.org/10.1134/S0006297909080100

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  • DOI: https://doi.org/10.1134/S0006297909080100

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