Abstract
Endopolyphosphatase activity has been revealed in cytosol preparations of the yeast Saccharomyces cerevisiae with inactivated PPX1 and PPN1 genes encoding exopolyphosphatases. The enzyme cleaves inorganic polyphosphates with chain length of 15 to 208 phosphate residues to shorter chains without the release of orthophosphate (Pi). The long chain polyphosphates are cleaved with preference over the short ones. Heparin, a known inhibitor of exopolyphosphatases, represses this activity. The endopolyphosphatase activity is not stimulated by Mg2+ or Co2+, in contrast to exopolyphosphatases. This activity along with a pyrophosphatase is supposed to be responsible for polyphosphate utilization as a phosphate reserve in a mutant devoid of exopolyphosphatases.
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Published in Russian in Biokhimiya, 2009, Vol. 74, No. 8, pp. 1037–1041.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM09-009, May 10, 2009.
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Lichko, L.P., Kulakovskaya, T.V., Kulakovskaya, E.V. et al. Finding of endopolyphosphatase activity in the yeast Saccharomyces cerevisiae . Biochemistry Moscow 74, 842–845 (2009). https://doi.org/10.1134/S0006297909080045
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DOI: https://doi.org/10.1134/S0006297909080045