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Study of the inhibitory effect of fatty acids on the interaction between DNA and polymerase β

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Abstract

The binding of human DNA polymerase β (pol β) to DNA template-primer duplex and single-stranded DNA in the absence or presence of pol β inhibitors has been studied using a surface plasmon resonance biosensor. Two fatty acids, linoleic acid and nervonic acid, were used as potent pol β inhibitors. In the interaction between pol β and DNA, pol β could bind to ssDNA in a single binding mode, but bound to DNA template-primer duplexes in a parallel mode. Both pol β inhibitors prevented the binding of pol β to the single strand overhang and changed the binding from parallel to single mode. The affinities of pol β to the template-primer duplex region in the presence of nervonic acid or linoleic acid were decreased by 20 and 5 times, respectively. The significant inhibitory effect of nervonic acid on the pol β-duplex interaction was due to both a 2-fold decrease in the association rate and a 9-fold increase in the dissociation rate. In the presence of linoleic acid, no significant change of association rate was observed, and the decrease in binding affinity of pol β to DNA was mainly due to 7-fold increase in the dissociation rate.

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Abbreviations

LA:

linoleic acid

NA:

nervonic acid

pol β:

DNA polymerase β

SPR:

surface plasmon resonance

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Authors and Affiliations

  1. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education; Bioengineering College, Chongqing University, Chongqing, 400030, China

    Jun Yang, Jing Yang, Jing Xu, Ning Hu, Min Wang, Yuan-Yi Li, Lei Zhan, Song Wu & Xiao-Lin Zheng

  2. Department of Information Engineering, Chongqing Communication College, Chongqing, 400035, China

    Jing Yang

  3. Southwest Eye Hospital, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

    Zheng-Qin Yin

  4. Kharkov National University of Radioelectronics, UA-61166, Kharkov, Ukraine

    I. Svir

Authors
  1. Jun Yang
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  2. Jing Yang
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  3. Zheng-Qin Yin
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  4. Jing Xu
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  5. Ning Hu
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  7. Min Wang
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  9. Lei Zhan
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  10. Song Wu
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  11. Xiao-Lin Zheng
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Corresponding authors

Correspondence to Jun Yang or Zheng-Qin Yin.

Additional information

Published in Russian in Biokhimiya, 2009, Vol. 74, No. 7, pp. 1000–1006.

These authors contributed equally.

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Yang, J., Yang, J., Yin, ZQ. et al. Study of the inhibitory effect of fatty acids on the interaction between DNA and polymerase β. Biochemistry Moscow 74, 813–818 (2009). https://doi.org/10.1134/S0006297909070165

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  • DOI: https://doi.org/10.1134/S0006297909070165

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