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Effect of solvent phase transitions on enzymatic activity and structure of laccase from Coriolus hirsutus

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Abstract

The effect of solvent phase transitions on catalytic activity and structure of the active site of laccase produced by the Basidiomycetes Coriolus hirsutus 072 was studied. As shown by small-angle X-ray scattering, laccase exists in solution as a mixture of monomeric and aggregated particles in the percent ratio 85: 15. This ratio did not change on phase transitions. A complex nature of laccase activity dynamics during thawing and further heating to 20°C was shown. Spontaneous oxidation of T1 copper center in the temperature range 12–20°C was not observed. According to spectral data, the structure of laccase active sites including all copper centers of types T1, T2, and T3 changes during the phase transition.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071, Moscow, Russia

    E. V. Stepanova, T. V. Fedorova & O. V. Koroleva

  2. Emmanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119334, Moscow, Russia

    O. N. Sorokina

  3. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninsky pr. 59, 119333, Moscow, Russia

    V. V. Volkov & A. T. Dembo

Authors
  1. E. V. Stepanova
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  2. T. V. Fedorova
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  3. O. N. Sorokina
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  4. V. V. Volkov
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  5. O. V. Koroleva
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  6. A. T. Dembo
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Correspondence to O. V. Koroleva.

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Original Russian Text © E. V. Stepanova, T. V. Fedorova, O. N. Sorokina, V. V. Volkov, O. V. Koroleva, A. T. Dembo, 2009, published in Biokhimiya, 2009, Vol. 74, No. 4, pp. 476–485.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-245, December 28, 2008.

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Stepanova, E.V., Fedorova, T.V., Sorokina, O.N. et al. Effect of solvent phase transitions on enzymatic activity and structure of laccase from Coriolus hirsutus . Biochemistry Moscow 74, 385–392 (2009). https://doi.org/10.1134/S0006297909040051

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  • DOI: https://doi.org/10.1134/S0006297909040051

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