Abstract
Novel trypsin inhibitors from the white rot fungus Abortiporus biennis were isolated, partially purified, and char- acterized. The inhibitors were purified by heat treatment, anion-exchange chromatography, and gel filtration. SDS-PAGE of the purified preparation demonstrated the presence of two proteins with molecular masses of 20 and 21.5 kDa. The A. biennis inhibitors were most active against trypsin, while chymotrypsin α, proteinase K, and Carlsberg subtilisin were inhibited to a smaller extent. The inhibitors are acidic proteins with remarkably high heat stability.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AMC:
-
7-amino-4-methylcoumarin
References
Rawlings, N. D., Tolle, D. P., and Barrett, A. J. (2004) Biochem. J., 378, 705–716.
Bode, W., and Huber, R. (2000) Biochim. Biophys. Acta, 1477, 241–252.
Bode, W., and Huber, R. (1992) Eur. J. Biochem., 204, 433–451.
Krowarsch, D., Cierpicki, T., Jelen, F., and Otlewski, J. (2003) Cell. Mol. Life Sci., 60, 2427–2444.
Gettins, P. G. W. (2002) Chem. Rev., 102, 4751–4803.
Schwarze, F. W. M. R., Engels, J., and Mattheck, C. (2000) Fungal Strategies of Wood Decay in Trees, Springer-Verlag, Berlin-Heidelberg.
Shah, V., and Nerud, F. (2002) Can. J. Microbiol., 48, 857–870.
Lindeberg, G., and Holm, G. (1952) Physiol. Plant., 5, 100–114.
Lee, T.-M., and Lin, Y.-H. (1995) Plant Sci., 106, 43–54.
Laemmli, U. K. (1970) Nature, 227, 680–685.
Uriel, J., and Berges, J. (1968) Nature, 218, 578–580.
Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. (1996) Anal. Chem., 68, 850–858.
Schacterle, G. R., and Pollack, R. L. (1973) Anal. Biochem., 51, 654–655.
Dohmae, N., Takio, K., Tsumuraya, Y., and Hashimoto, Y. (1995) Arch. Biochem. Biophys., 316, 498–506.
Odani, S., Tominaga, S., Kondou, S., Hori, H., Koide, T., Hara, S., Isemura, M., and Tsunasawa, S. (1999) Eur. J. Biochem., 262, 915–923.
Zuchowski, J., and Grzywnowicz, K. (2006) Curr. Microbiol., 53, 259–264.
Tian, Y.-P., and Zhang, K.-C. (2005) Enzyme Microb. Technol., 36, 357–361.
Brzin, J., Rogelj, B., Popovic, T., Strukelj, B., and Ritonja, A. (2000) J. Biol. Chem., 275, 20104–20109.
Gzogyan, L. A., Proskuryakov, M. T., Ievleva, E. V., and Valueva, T. A. (2005) Appl. Biochem. Microbiol., 41, 538–541.
Doljak, B., Stegnar, M., Urleb, U., Kreft, S., Umek, A., Ciglaric, M., Strukelj, B., and Popovic, T. (2001) Blood Coagul. Fibrinolysis, 12, 123–128.
Mlinaric, A., Kreft, S., Umek, A., Strukelj, B., and Popovic, T. (2000) Acta Pharmacol., 50, 39–48.
Escudero, B., Parra, F., and Suarez-Rendueles, P. (1993) Biochimie, 75, 855–859.
Feldman, M. L., Oliva, C. R., Casalongue, C. A., and Daleo, G. R. (2000) Physiol. Plant., 109, 14–20.
Laskowski, M., Jr., and Kato, I. (1980) Annu. Rev. Biochem., 49, 593–625.
Schu, P., and Wolf, D. H. (1991) FEBS Lett., 283, 78–84.
Schu, P., Suarez-Rendueles, P., and Wolf, D. H. (1991) Eur. J. Biochem., 197, 1–7.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Biokhimiya, 2009, Vol. 74, No. 2, pp. 278–283.
Rights and permissions
About this article
Cite this article
Zuchowski, J., Jaszek, M. & Grzywnowicz, K. Novel trypsin inhibitors from the white rot fungus Abortiporus biennis. Partial purification and characterization. Biochemistry Moscow 74, 226–230 (2009). https://doi.org/10.1134/S0006297909020151
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297909020151