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Study of spatial organization of chicken α-globin gene domain by 3c technique

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Abstract

This work deals with 3C (Chromosome Conformation Capture) analysis of the chicken α-globin gene domain in embryonic erythrocytes and lymphoid cells. Ligation products were quantitatively analyzed by real-time PCR with TaqMan probes. It was found that in lymphoid cells, where α-globin gene is not active, the domain has a relatively extended configuration. In embryonic erythrocytes that transcribe αD and αA genes, simultaneous interaction of several domain elements was revealed including the major regulatory element, the erythroid-specific DNase I hypersensitive site at a distance of 9 kb upstream from the α-globin gene cluster (-9 DHS), promoter of the housekeeping gene CGTHBA, the αD-globin gene promoter, and the erythroid-specific enhancer located after the α-globin gene cluster. We suppose that such interaction is necessary to provide for the active transcription status of the chicken α-globin gene domains in erythroid cells.

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Abbreviations

BAC:

bacterial artificial chromosome

3C:

chromosome conformation capture

DHS:

DNase I hypersensitive sites

MRE:

major regulatory element

YAC:

yeast artificial chromosome

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, ul. Vavilova 34/5, 119334, Moscow, Russia

    A. A. Gavrilov & S. V. Razin

  2. Biological Faculty, Lomonosov Moscow State University, 119991, Moscow, Russia

    S. V. Razin

Authors
  1. A. A. Gavrilov
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  2. S. V. Razin
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Correspondence to A. A. Gavrilov.

Additional information

Original Russian Text © A. A. Gavrilov, S. V. Razin, 2008, published in Biokhimiya, 2008, Vol. 73, No. 11, pp. 1486–1494.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-214, October 5, 2008.

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Gavrilov, A.A., Razin, S.V. Study of spatial organization of chicken α-globin gene domain by 3c technique. Biochemistry Moscow 73, 1192–1199 (2008). https://doi.org/10.1134/S0006297908110047

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  • DOI: https://doi.org/10.1134/S0006297908110047

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