Abstract
The pathogenesis of cataract is associated with oxidative stress and with altered crystallin expression but it is still understood incompletely. In this study, the senescence-accelerated OXYS rats were used as a model. The first biomicro-scopic signs of cataract in OXYS rats were registered at the age of 1.5 months; at 3 months morbidity reached 90%, and at 6 months it reached 100%. Cataract manifestation progresses: at 24 months mature cataract was detected in 90% of eyes of OXYS rats, whereas in 80% of Wistar rat eyes only initial signs of this disease were detected. Analysis of lens redox-parameters has shown that in OXYS rats the intensity of tryptophan fluorescence is higher, the GSH content being higher at 2 months but during formation of mature cataract at 13, 18, and 24 months being lower than in Wistar rats. Decrease in solubility of OXYS rat lens proteins was observed at the age of 13 months. At the age of 3 months gene expression of αA-crystallin and αB-crystallin was 3-fold and 25% lower, respectively, than in Wistar rats. At the age of 14 months there was a 27-fold decrease in expression of αB-crystallin in OXYS rats and it became 21-fold lower than in control. Proteins are synthesized in lens epithelial cells and dystrophic changes in senile cataract result in decrease in structural protein expression. The changes observed in OXYS rats are evidently associated with the dystrophic changes in lens epithelium, which we have described earlier, and are consistent with the model of senile cataract.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- LPO:
-
lipid peroxidation
- MDA:
-
malondialdehyde
References
Truscott, R. J. (2006) in Prevention and Treatment of Age-Related Diseases (Rattan, S., and Kassem, M., eds.) Springer, N.Y., pp. 159–174.
Shinohara, T., White, H., Mulhern, M. L., and Maisel, H. (2007) Medical Hyp., 69, 669–677.
Shang, F., Nowell, T., Jr., Gong, X., Smith, D. E., Dallal, G. E., Khu, P., and Taylor, A. (2002) Exp. Eye Res., 5, 109–111.
Nishimoto, H., Uga, S., Miyata, M., Ishikawa, S., and Yamashita, K. (1993) Graefes Arch. Clin. Exp. Ophthalmol., 231, 722–728.
Noriaki, N., Noriko, T., Akira, K., and Yoshimasan, I. (2006) Biol. Pharm. Bull., 29, 236–248.
Mori, M., Li, G., and Abe, I. (2006) J. Clin. Invest., 116, 395–404.
Solov’eva, N. A., Morozkova, T. S., and Salganik, R. I. (1975) Genetika, 11, 63–71.
Kolosova, N. G., Lebedev, P. A., Aidagulova, S. V., and Morozkova, T. S. (2003) Byul. Eksp. Biol. Med., 136, 467–471.
Kolosova, N. G., Lebedev, P. A., Fursova, A. Zh., Morozkova, T. S., and Gusarevich, O. G. (2003) Usp. Gerontol., 12, 143–148.
Marsili, S., Salganik, R. I., Albright, C. D., Freel, C. D., Johnsen, S., Peiffer, R. L., and Costello, M. J. (2004) Exp. Eye Res., 79, 595–612.
Gusarevich, O. G., Kolosova, N. G., and Fursova, A. Zh. (2005) Rus. Med. Zh., 4, 186–189.
Kolosova, N. G., Trofimova, N. A., Shcheglova, T. V., and Sergeeva, S. V. (2005) Byul. Eksp. Biol. Med., 139, 397–999.
Bobko, A. A., Sergeeva, S. V., Bagryanskaya, E. G., Markel, A. L., Khramtsov, V. V., Reznikov, V. A., and Kolosova, N. G. (2005) Biochem. Biophys. Res. Commun., 330, 367–370.
Sergeeva, S. V., Bagryanskaya, E. G., Korbolina, E. E., and Kolosova, N. G. (2006) Exp. Gerontol., 41, 141–150.
Kolosova, N. G., Shcheglova, T. V., Sergeeva, S. V., and Loskutova, L. V. (2006) Neurobiol. Aging, 27, 1289–1297.
Tsentalovich, Y. P., Snytnikova, O. A., Forbes, M. D. E., Chernyak, E. I., and Morozov, S. V. (2006) Exp. Eye Res., 83, 1439–1445.
Hawse, J. R., Hejtmancik, J. F., Huang, Q., Sheets, N. L., Hosack, D. A., Lempicki, R. A., Horwitz, J., and Kantorow, M. (2003) Molecular Vision, 9, 515–537.
Babizhayev, M. A., Deyev, A. I., Yermakova, V. N., Brikman, I. V., and Bours, J. (2004) Drugs R. D., 5, 125–139.
Ellman, G. L. (1959) Arch. Biochem. Biophys., 82, 70–77.
Esterbauer, H., and Cheeseman, K. H. (1990) Meth. Enzymol., 186, 407–420.
Nolan, T., Hands, R. E., and Bustin, S. A. (2006) Nature Protocols, 1, 1559–1582.
Vinson, J. A. (2006) Pathophysiology, 13, 151–162.
Kolosova, N. G., Fursova, A. Zh., Lebedev, P. A., and Gusarevich, O. G. (2004) Oftalmol. Zh., 3, 237–242.
Aseychev, A. V., Sitarchuk, I. A., Deev, A. I., Aitmagambetov, M. T., and Vladimirov, Yu. A. (1994) in Study of Kinetics of Accumulation and Molecular Mass of Lens Fluorophores during Periods Preceding Cataract Development (Tikhonov, A. N., Sadovnichiy, V. A., et al., eds.) MSU Press, Moscow, pp. 22–27.
Deyev, A. I., Aseychev, A. V., Sitartchuk, I. A., Fedorenko, B. S., and Vladimirov, Yu. A. (1999) in Ocular Radiation Risk Assessment in Population Exposed to Environmental Radiation Contamination (Junk, A. K., et al., eds.) Kluwer Academic Publishers, Dorbrecht-Boston-London, pp. 81–88.
Ferrer, V., Gasca, E., Sastre, J., and Pallardo, F. V. (1990) Biochem. J., 269, 531–534.
Andley, U. P. (2007) Progr. Retinal Eye Res., 26, 78–98.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © Yu. V. Rumyantseva, A. Zh. Fursova, L. A. Fedoseeva, N. G. Kolosova, 2008, published in Biokhimiya, 2008, Vol. 73, No. 11, pp. 1467–1475.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-133, October 12, 2008.
Rights and permissions
About this article
Cite this article
Rumyantseva, Y.V., Fursova, A.Z., Fedoseeva, L.A. et al. Changes in physicochemical parameters and α-crystallin expression in the lens during cataract development in OXYS rats. Biochemistry Moscow 73, 1176–1182 (2008). https://doi.org/10.1134/S0006297908110023
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297908110023