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Brain cathepsin B cleaves a caspase substrate

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Abstract

We show that an enzyme exists in rat brain capable of cleaving the caspase-3 specific peptide substrate Ac-DEVD-AMC at low pH. The enzyme shows properties of a cysteine protease and is localized, predominantly, in lysosomes. We have purified this enzyme from rat brain and identified it by MALDI-TOF MS. The enzyme possessing “acidic” DEVDase activity in rat brain appears to be cathepsin B. It remains obscure, whether cathepsin B participates in cleavage of caspase-3 substrates in vivo. We suggest that under certain conditions (e.g. in hypoxia) cathepsin B participates in cleavage of caspase-3 substrates in brain cells.

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Abbreviations

AMC:

7-amino-4-methylcoumarin

CA-074:

N-[L-3-trans-(propylcarbamoyl)oxirane-2-carbonyl]-L-isoleucyl-L-proline

DTT:

dithiothreitol

MALDI-TOF MS:

matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, ul. Butlerova 5a, 117485, Moscow, Russia

    A. A. Yakovlev, M. V. Onufriev & N. V. Gulyaeva

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, 142290, Pushchino, Moscow Region, Russia

    A. A. Yakovlev & I. P. Beletsky

  3. Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, pr. Nauki 6, 142290, Pushchino, Moscow Region, Russia

    A. Yu. Gorokhovatsky

Authors
  1. A. A. Yakovlev
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  2. A. Yu. Gorokhovatsky
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  3. M. V. Onufriev
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  4. I. P. Beletsky
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  5. N. V. Gulyaeva
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Corresponding author

Correspondence to N. V. Gulyaeva.

Additional information

Published in Russian in Biokhimiya, 2008, Vol. 73, No. 3, pp. 408–413.

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Yakovlev, A.A., Gorokhovatsky, A.Y., Onufriev, M.V. et al. Brain cathepsin B cleaves a caspase substrate. Biochemistry Moscow 73, 332–336 (2008). https://doi.org/10.1134/S0006297908030140

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  • DOI: https://doi.org/10.1134/S0006297908030140

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