Abstract
Heavy fragments were isolated from pea chloroplasts using digitonin treatment and differential centrifugation. The particles were characterized by a significantly lowered chlorophyll a/b ratio, contents of photosystem I (PS I) proteins and ATPase, as well as of amount of P700. The content of photosystem II (PS II) proteins decreased insignificantly, whereas that of proteins of the light-harvesting complex II did not change. The absorption and low-temperature fluorescence spectra were indicative of a decreased content of PS I. Electron microscopy of ultrathin sections of heavy fragment preparations identified them as grana with reduced content of thylakoids. The diameter of these particles was practically the same as within chloroplasts. Comparison of various characteristics of the fragments and chloroplasts from which the fragments were isolated allowed us to define a high degree of preservation of marginal regions in thylakoids present in the heavy fragment particles. Analysis of the results shows that the procedure of fragmentation produces grana with high extent of thylakoid integrity. The phenomenon of reduction of the thylakoid content in grana, occurring as our heavy fragments, is considered in the frame of our previous hypothesis concerning the peculiarities of grana organization in the transversal direction.
Similar content being viewed by others
Abbreviations
- Chl:
-
chlorophyll
- LHC II:
-
light-harvesting complex II
- PS I, PS II:
-
photosystems I and II
References
Rozak, P. R., Seiser, R. M., Wacholtz, W. F., and Wise, R. R. (2002) Plant Cell Environ., 25, 421–429.
Sharkova, V. E., and Bubolo, L. S. (1996) Fiziol. Rast., 43, 409–417.
Wollenberger, L., Stefansson, H., Yu, S.-G., and Albertsson, P.-A. (1994) Biochim. Biophys. Acta, 1184, 93–102.
Andreasson, E., Svensson, P., Weibull, C., and Albertsson, P.-A. (1988) Biochim. Biophys. Acta, 936, 339–350.
Kochubey, S. M., Shevchenko, V. V., and Bondarenko, O. Yu. (2005) Dokl. NAN Ukr., 4, 161–166.
Laemmli, U. K. (1970) Nature, 292, 200–202.
Arnon, D. I. (1949) Plant. Physiol., 24, 1–15.
Vernon, L. R. (1960) Anal. Chem., 32, 1144–1150.
Volovik, O. I., Korneev, D. Yu., Porubleva, L. V., and Shevchenko, V. V. (2003) Fiziol. Rast., 50, 325–331.
Kochubey, S. M., Vovk, A. I., Bondarenko, O. Yu., Shevchenko, V. V., Bugas, R. V., Mel’nik, A. K., and Tanchuk, V. Yu. (2007) Biochemistry (Moscow), 72, 558–564.
Albertsson, P.-A. (1995) Photosynth. Res., 46, 141–149.
Albertsson, P.-A. (2001) Trends Plant Sci., 6, 349–354.
Kochubey, S. M., Shevchenko, V. V., and Bondarenko, O. Yu. (2003) Fiziol. Rast., 50, 325–331.
Kochubey, S. M., Shevchenko, V. V., and Korneev, D. Yu. (2007) Structural Organization and Functional Peculiarities of the Light Phase of Photosynthesis [in Russian], Logos, Kiiv.
Kochubey, S. M., Shevchenko, V. V., and Bondarenko, O. Yu. (2005) Fiziol. Rast., 52, 499–506.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S. M. Kochubey, O. Yu. Bondarenko, V. V. Shevchenko, 2007, published in Biokhimiya, 2007, Vol. 72, No. 9, pp. 1255–1261.
Rights and permissions
About this article
Cite this article
Kochubey, S.M., Bondarenko, O.Y. & Shevchenko, V.V. A new type of subchloroplast fragments isolated from pea chloroplasts in the presence of digitonin. Biochemistry Moscow 72, 1021–1026 (2007). https://doi.org/10.1134/S0006297907090155
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1134/S0006297907090155