Abstract
The pH dependences of activities of homogenous hydrogenases of Thiocapsa roseopersicina and Desulfomicrobium baculatum in the reaction of hydrogen uptake in solution in the presence of benzyl viologen and the pH dependences of catalytic currents of hydrogen oxidation by electrodes on which these hydrogenases were immobilized were compared. Maximal activities of the hydrogenases from T. roseopersicina and D. baculatum in the reaction hydrogen uptake in solution were observed at pH 9.5 and 8.5, respectively. However, the steady-state current caused by catalytic uptake of hydrogen was maximal for the T. roseopersicina hydrogenase-containing electrode at pH 5.5–6.5 under overvoltage of 30–60 mV, whereas for electrodes with D. baculatum hydrogenase it was maximal at pH 6.0–6.5. Analysis of these data suggests that pH-dependent changes in the hydrogenase activities in solution during hydrogen uptake are due not only to the effect of proton concentration on the enzyme conformation or protonation of certain groups of the enzyme active center, but they are rather indicative of changes in free energy of the reaction accompanying changes in pH.
Similar content being viewed by others
References
Fersht, E. (1980) Structure and Mechanism of Enzyme Action [Russian translation], Mir, Moscow.
Adams, M. W. W., Mortenson, L., and Chen, J. S. (1981) Biochim. Biophys. Acta, 594, 105–176.
Zorin, N. A., Dimon, B., Gagnon, J., Gaillard, J., Carrier, P., and Vignais, P. M. (1996) Eur. J. Biochem., 241, 675–681.
Serebryakova, L. T., and Sheremetieva, M. E. (2006) Biochemistry (Moscow), 71, 1370–1376.
Schink, B., and Schlegel, H. G. (1979) Biochim. Biophys. Acta, 567, 315–324.
Arp, D. J., and Burris, R. H. (1979) Biochim. Biophys. Acta, 570, 221–230.
Lespinat, P. A., Berlier, Y., Fauque, G., Czechowcky, M., Dimon, B., and LeGall, J. (1986) Biochimie, 68, 55–61.
Vignais, P. M., Cournac, L., Hanchikian, E. C., Elsen, S., Serebryakova, L. T., Zorin, N. A., and Dimon, B. (2002) Int. J. Hydrogen Energy, 27, 1441–1448.
Varfolomeyev, S. D., Yaropolov, A. I., and Karyakin, A. A. (1993) J. Biotechnol., 27, 331–339.
Zadvorny, O. A., Zorin, N. A., and Gogotov, I. N. (2000) Biochemistry (Moscow), 65, 1287–1291.
Patil, D. (1994) Meth. Enzymol., 243, 68.
Morozov, S. V., Voronin, O. G., Karyakina, E. E., Zorin, N. A., Cosnier, S., and Karyakin, A. A. (2006) Electrochem. Commun., 8, 851–854.
Zorin, N. A. (1986) Biochimie, 68, 97–101.
Laurinavichene, T. V., Zorin, N. A., and Tsygankov, A. A. (2002) Arch. Microbiol., 178, 437–442.
Dobos, D. (1975) Electrochemical Data, Akademiai Kiado, Budapest.
Bishop, E. (1976) Indicators [Russian translation], Vol. 2, Mir, Moscow.
Sim, E., and Vignais, P. M. (1979) Biochim. Biophys. Acta, 570, 43–55.
Knuttel, K., Schneider, K., Schlegel, H. G., and Muller, A. (1989) Eur. J. Biochem., 179, 101–108.
Coremans, J. M. C. C., van der Zwaan, J. W., and Albracht, S. P. J. (1992) Biochim. Biophys. Acta, 1119, 157–168.
Gitlitz, P. H., and Krasna, A. I. (1975) Biochemistry, 14, 2561–2567.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.A. Tsygankov, E.A. Minakov, N.A. Zorin, K.S. Gosteva, O.G. Voronin, A.A. Karyakin, 2007, published in Biokhimiya, 2007, Vol. 72, No. 9, pp. 1189–1195.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM07-114, August 19, 2007.
Rights and permissions
About this article
Cite this article
Tsygankov, A.A., Minakov, E.A., Zorin, N.A. et al. Measuring the pH dependence of hydrogenase activities. Biochemistry Moscow 72, 968–973 (2007). https://doi.org/10.1134/S0006297907090076
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1134/S0006297907090076