Abstract
Destruction of guard cell nuclei in epidermis isolated from leaves of pea, maize, sunflower, and haricot bean, as well as destruction of cell nuclei in leaves of the aquatic plants waterweed and eelgrass were induced by cyanide. Destruction of nuclei was strengthened by illumination, prevented by the antioxidant α-tocopherol and an electron acceptor N,N,N′,N′-tetramethyl-p-phenylenediamine, and removed by quiacrine. Photosynthetic O2 evolution by the leaf slices of a C3 plant (pea), or a C4 plant (maize) was inhibited by CN− inactivating ribulose-1,5-bisphosphate carboxylase, and was renewed by subsequent addition of the electron acceptor p-benzoquinone.
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Abbreviations
- BQ:
-
p-benzoquinone
- DCF:
-
2′, 7′-dichlorofluorescein
- DCFH:
-
2′, 7′-dichlorofluorescin
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1, 1-dimethylurea
- EC:
-
basal epidermal cells
- FeCy:
-
potassium ferricyanide
- GC:
-
stoma guard cells
- PCD:
-
programmed cell death
- ROS:
-
reactive oxygen species
- Rubisco:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- TMPD:
-
N,N,N′,N′-tetramethyl-p-phenylenediamine
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Original Russian Text © L. A. Vasil’ev, A. A. Vorobyov, E. V. Dzyubinskaya, A. V. Nesov, A. A. Shestak, V. D. Samuilov, 2007, published in Biokhimiyo, 2007, Vol. 72, No. 5, pp. 707–713.
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Vasil’ev, L.A., Vorobyov, A.A., Dzyubinskaya, E.V. et al. Cyanide-induced death of cells in plant leaves. Biochemistry Moscow 72, 572–577 (2007). https://doi.org/10.1134/S000629790705015X
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DOI: https://doi.org/10.1134/S000629790705015X