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Influence of ultraviolet-C on structure and function of Synechococcus sp. PCC 7942 photolyase

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Abstract

In this work, an over-expressed cyclobutane pyrimidine dimer (CPD) photolyase of Synechococcus sp. PCC 7942 was used to investigate UV-C (ultraviolet irradiation of C-region) influence on photoreactivation. In vivo photoreactivation experiments indicated that the survival rate decreased from 100 to 2.6% when the UV-C flux was increased from 1.1 to 68.5 μW/cm2. It seemed that the photolyase was easily inactivated at UV-C intensities ≥25.5 μW/cm2. Spectrometric analysis indicated that tertiary structure of the photolyase changed evidently when the UV-C fluxes were ≥25.5 μW/cm2, while the secondary structure was almost unchanged even at 170 μW/cm2. Band shift assay indicated that catalytic activity of the photolyase was impaired at fluxes ≥25.5 μW/cm2, but no significant influence on DNA-binding activity was observed. These results suggest that photoreactivation is efficient at UV-C fluxes ≤25.5 μW/cm2, but would be impaired by intense UV-C irradiation due to structure changes of the photolyase.

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Abbreviations

CPD:

cyclobutane pyrimidine dimer

IPTG:

isopropyl-thio-β-D-galactoside

UV-A, UV-B, UV-C:

ultraviolet irradiation of A-, B-, and C-region, respectively

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, P. R. China

    L. W. Zhang, M. Li & Q. Y. Wu

Authors
  1. L. W. Zhang
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  2. M. Li
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  3. Q. Y. Wu
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Corresponding author

Correspondence to Q. Y. Wu.

Additional information

Published in Russian in Biokhimiya, 2007, Vol. 72, No. 5, pp. 668–673.

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Zhang, L.W., Li, M. & Wu, Q.Y. Influence of ultraviolet-C on structure and function of Synechococcus sp. PCC 7942 photolyase. Biochemistry Moscow 72, 540–544 (2007). https://doi.org/10.1134/S0006297907050100

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  • DOI: https://doi.org/10.1134/S0006297907050100

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