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Mouse retinal progenitor cell (RPC) cocultivation with retinal pigment epithelial cell culture affects features of RPC differentiation

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Abstract

We provide evidence that coculturing of retinal progenitor cells (RPC) with retinal pigment epithelial cells significantly biases the standard in vitro RPC differentiation patterns. In particular, in cocultivation experiments RPCs lost the ability to differentiate spontaneously and displayed ∼2.1–2.4-fold increase in immunoreactivity to the neural stem cell marker nestin and ∼1.6–1.7-fold increase in rod photoreceptor cell rhodopsin marker immunoreactivity. The data suggest the influence of the intercellular interaction networks on RPC differentiation.

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Abbreviations

bFGF:

basic fibroblast growth factor

DIL:

1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate

EGF:

epidermal growth factor

FCS:

fetal calf serum

GFAP:

glial fibrillary acidic protein

NGF:

nerve growth factor

NT3:

neurotrophin-3

PDGF:

platelet-derived growth factor

RPC:

retinal progenitor cell

RPE:

retinal pigment epithelial cell

RA:

all-trans retinoic acid

TGFα:

transforming growth factor α

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklayạ 16/10, 117997, Moscow, Russia

    I. V. Kholodenko, A. A. Buzdin, R. V. Kholodenko, J. A. Baibikova & E. D. Sverdlov

  2. Russian State Medical University, ul. Ostrovityanova 1, 117312, Moscow, Russia

    V. F. Sorokin & V. N. Yarygin

Authors
  1. I. V. Kholodenko
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  2. A. A. Buzdin
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  3. R. V. Kholodenko
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  4. J. A. Baibikova
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  5. V. F. Sorokin
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  6. V. N. Yarygin
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  7. E. D. Sverdlov
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Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 7, pp. 945–953.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM06-005, May 14, 2006.

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Kholodenko, I.V., Buzdin, A.A., Kholodenko, R.V. et al. Mouse retinal progenitor cell (RPC) cocultivation with retinal pigment epithelial cell culture affects features of RPC differentiation. Biochemistry (Moscow) 71, 767–774 (2006). https://doi.org/10.1134/S0006297906070091

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  • DOI: https://doi.org/10.1134/S0006297906070091

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