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Does a deficiency of the signal recognition particle (SRP)-pathway affect the biosynthesis of its components in Saccharomyces cerevisiae and Escherichia coli?

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Abstract

We studied the behavior of the signal recognition particle (SRP) components in Saccharomyces cerevisiae upon deficiencies of the protein transport caused by the absence of the SRP membrane receptor α-subunit. A decrease in the concentration of the SRP membrane receptor α-subunit in the cell significantly decreased the level of an SRP component, protein SRP72, as well as the levels of mRNAs of SRP protein components and the SRP receptor β-subunit. But the amount of 7SL RNA remained unchanged. In contrast, in Escherichia coli cells the gradual decrease in the level of the protein FtsY (a homolog of the SRP membrane receptor α-subunit) was not associated with changes in the Ffh protein level.

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Abbreviations

SRP:

signal recognition particle

SR:

SRP-receptor

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Authors and Affiliations

  1. Faculty of Chemistry, Lomonosov Moscow State University, 119992, Moscow, Russia

    O. N. Kovalskaya, P. V. Sergiev, A. A. Bogdanov & O. A. Dontsova

Authors
  1. O. N. Kovalskaya
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  2. P. V. Sergiev
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  3. A. A. Bogdanov
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  4. O. A. Dontsova
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Corresponding author

Correspondence to O. N. Kovalskaya.

Additional information

Original Russian Text © O. N. Kovalskaya (O. N. Avdeeva), P. V. Sergiev, A. A. Bogdanov, O. A. Dontsova, 2006, published in Biokhimiya, 2006, Vol. 71, No. 7, pp. 894–901.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM06-074, June 11, 2006.

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Kovalskaya, O.N., Sergiev, P.V., Bogdanov, A.A. et al. Does a deficiency of the signal recognition particle (SRP)-pathway affect the biosynthesis of its components in Saccharomyces cerevisiae and Escherichia coli?. Biochemistry (Moscow) 71, 723–729 (2006). https://doi.org/10.1134/S0006297906070042

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  • DOI: https://doi.org/10.1134/S0006297906070042

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