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Two methods for determination of transketolase activity

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Abstract

Two new optical methods for transketolase activity assay using only one substrate, xylulose 5-phosphate or glycol aldehyde, have been developed. For transketolase activity assay in the first method, it is necessary to add auxiliary enzyme, glyceraldehyde phosphate dehydrogenase. It is not needed in the second method. The range of transketolase concentration in the activity assay is 0.036–0.144 U/ml for the first method and 1.8–6.8 U/ml for the second one.

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References

  1. Kochetov, G. A. (1982) Meth. Enzymol., 90, 209–223.

    Article  PubMed  CAS  Google Scholar 

  2. Schenk, G., Duggleby, R. G., and Nixon, P. F. (1998) Int. J. Biochem. Cell Biol., 30, 1297–1318.

    Article  PubMed  CAS  Google Scholar 

  3. Schneider, G., and Lindqvist, Y. (1998) Biochim. Biophys. Acta, 1385, 387–398.

    PubMed  CAS  Google Scholar 

  4. Horecker, R. L. (2002) J. Biol. Chem., 277, 47965–47971.

    Article  PubMed  CAS  Google Scholar 

  5. Usmanov, R. A., and Kochetov, G. A. (1981) Biochem. Int., 3, 33–39.

    CAS  Google Scholar 

  6. Kuimov, A. N., Kovina, M. V., and Kochetov, G. A. (1988) Biochem. Int., 17, 517–521.

    PubMed  CAS  Google Scholar 

  7. Teige, M., Melzer, M., and Sus, K.-H. (1998) Eur. J. Biochem., 252, 237–244.

    Article  PubMed  CAS  Google Scholar 

  8. Tikhomirova, N. K., Merchan, A. Y., and Kochetov, G. A. (1990) FEBS Lett., 274, 27–29.

    Article  PubMed  CAS  Google Scholar 

  9. Wikner, Ch., Nilsson, U., Meshalkina, L., Udekwu, C., Lindqvist, Y., and Schneider, G. (1997) Biochemistry, 36, 15643–15649.

    Article  PubMed  CAS  Google Scholar 

  10. Usmanov, R. A., and Kochetov, G. A. (1982) Biochem. Int., 5, 727–734.

    CAS  Google Scholar 

  11. Usmanov, R. A., and Kochetov, G. A. (1983) Biokhimyia, 48, 550–558.

    CAS  Google Scholar 

  12. Tikhomirova, N. K., and Kochetov, G. A. (1990) Biochem. Int., 22, 31–36.

    Article  PubMed  CAS  Google Scholar 

  13. Bykova, I. A., Solov’eva, O. N., Meshalkina, L. E., Kovina, M. V., and Kochetov, G. A. (2001) Biochem. Biophys. Res. Commun., 280, 845–847.

    Article  PubMed  CAS  Google Scholar 

  14. Sevostyanova, I. A., Solovjeva, O. N., and Kochetov, G. A. (2004) Biochem. Biophys. Res. Commun., 313, 771–774.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    I. A. Sevostyanova, O. N. Solovjeva & G. A. Kochetov

Authors
  1. I. A. Sevostyanova
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  2. O. N. Solovjeva
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  3. G. A. Kochetov
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Corresponding author

Correspondence to G. A. Kochetov.

Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 5, pp. 693–696.

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Sevostyanova, I.A., Solovjeva, O.N. & Kochetov, G.A. Two methods for determination of transketolase activity. Biochemistry (Moscow) 71, 560–562 (2006). https://doi.org/10.1134/S0006297906050154

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  • DOI: https://doi.org/10.1134/S0006297906050154

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