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Radical scavenging activity of ribonuclease inhibitor from cow placenta

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Abstract

Cow placenta ribonuclease inhibitor (CPRI) has been purified 5062-fold by affinity chromatography, the product being homogeneous by sodium dodecyl sulfate-gel electrophoresis. The chemiluminescence technique was used to determine the radical scavenging activities of CPRI toward different reactive oxygen species (ROS) including superoxide anion (\(O\mathop \cdot \limits_2^ - \)), hydroxyl radical (OH.), lipid-derived radicals (R.), and singlet oxygen (1O2). CPRI could effectively scavenge \(O\mathop \cdot \limits_2^ - \), OH., R., and 1O2 at EC50 of 0.12, 0.008, 0.009, and 0.006 mg/ml, respectively. In addition, the radical scavenging activities of CPRI were higher than those of tea polyphenols, indicating that CPRI is a powerful antioxidant.

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Abbreviations

CL:

chemiluminescence

CPRI:

cow placenta ribonuclease inhibitor

DTT:

dithiothreitol

EC50 :

effective concentration for 50% inhibition

\(O\mathop \cdot \limits_2^ - \) :

superoxide anion

OH. :

hydroxyl radical

1O2:

singlet oxygen

RI:

ribonuclease inhibitor

RNase:

ribonuclease

ROS:

reactive oxygen species

R. :

lipid-derived radicals

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Authors and Affiliations

  1. Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300222, P. R. China

    Shuo Wang & Haiping Li

  2. Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300134, P. R. China

    Haiping Li

Authors
  1. Shuo Wang
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  2. Haiping Li
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Correspondence to Shuo Wang.

Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 5, pp. 645–650.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM05-199, February 26, 2006.

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Wang, S., Li, H. Radical scavenging activity of ribonuclease inhibitor from cow placenta. Biochemistry (Moscow) 71, 520–524 (2006). https://doi.org/10.1134/S0006297906050087

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  • DOI: https://doi.org/10.1134/S0006297906050087

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