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The Diatom Nanofrustulum shiloi As a Promising Species in Modern Biotechnology

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Abstract

The results of studies of an intensive culture of a new species of bentoplanktonic diatom N. shiloi (Lee, Reimer et McEnery) Round, Hallsteinsen et Paasche 1999 isolated from the Black Sea are presented. Detailed descriptions are provided of the methods used to isolate the species into an algologically pure culture and its morphological and taxonomic features under light and electron scanning microscopy. The biochemical and production characteristics were also studied including the ability of the strain to accumulate fucoxanthin (Fx) and polyunsaturated fatty acids (PUFA) in laboratory conditions. During the exponential growth phase, the specific culture growth rate was µ = 0.8 1/day, and the maximum productivity P = 0.46 g dry weight/(L day). The accumulation of PUFAs in the biomass of N. shiloi reached 67.39 mg/g dry weight of algae. The Fx concentration in the biomass at the beginning of the stationary growth phase was 10 mg/g dry weight. The fairly high rate of Fx biosynthesis in microalgae cells, as well as the composition of fatty acids of the Black Sea strain, makes it possible to classify N. shiloi as a promising object in biotechnology.

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ACKNOWLEDGMENTS

We would like to express our gratitude to V.N. Lishaev for the assistance in obtaining SEM images.

Funding

This research received financial support within the governmental research assignments no. 023032700554-2-1.6.16. “Comprehensive study of the functioning mechanisms of biotechnological complexes with the aim of obtaining active substances from aquatic organisms”, with partial financial support from the Russian Science Foundation (project no. 19-19-00083).

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Authors and Affiliations

  1. Federal Research Center Kovalevskii Institute of Biology of the Southern Seas, Russian Academy of Sciences, 299011, Sevastopol, Russia

    A. A. Blaginina, S. N. Zheleznova, E. S. Miroshnichenko, R. G. Gevorgiz & L. I. Ryabushko

  2. Kutateladze Institute of Thermophysics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    S. N. Zheleznova & R. G. Gevorgiz

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  1. A. A. Blaginina
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Contributions

Conceptualization S.Z.; R.G., L.R.; microalgae sample processing, preparation of diatom samples for SEM and LM and obtaining images, A.B.; microalgae species identification, L.R., A.B.; Isolation and establishing monoculture, A.B.; data and biochemical analysis, S.Z., R.G. All authors have read and agreed to the published version of the manuscript.

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Correspondence to A. A. Blaginina.

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Blaginina, A.A., Zheleznova, S.N., Miroshnichenko, E.S. et al. The Diatom Nanofrustulum shiloi As a Promising Species in Modern Biotechnology. Appl Biochem Microbiol 60, 483–495 (2024). https://doi.org/10.1134/S0003683824603615

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