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Decolorization of Crystal Violet by a Mixed Culture under the Influence of Bioelectrochemical Stimulation

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Abstract

A significant variation in the relative representation of copies of bacterial genes of dye-decolorizing DyP peroxidases typical for the genus Shewanella and a number of other microorganisms was found in the bottom sediments of freshwater reservoirs. It was found that the specific rate of decolorization of crystal violet in a laboratory bioelectrochemical system by a mixed culture of bottom sediments, which showed the highest representation of DyP genes, depended on the method of electrical stimulation of the external circuit and the concentration of the dye. After an increase in the concentration of more than 20 microns, the maximum speed was achieved in the presence of an ionistor polarly connected to the external electrical circuit of the bioelectrochemical system and amounted to 3.23 ± 0.11 μM/h, while with the opposite polarity connection, a minimum value of 2.07 ± 0.08 μM/h was observed. In the case of an open circuit and a resistor, similar indicators occurred with 2.88 ± 0.09 and 2.67 ± 0.12 μM/h, respectively. When analyzing the decolorization products, a consistent decrease in the maxima of the absorption bands of the dye was noted, indicating its more complete degradation by the mixed culture. The results may be of interest for the development of methods to improve the efficiency of bioelectrochemical methods of environmental biotechnology by electrostimulation of the external circuit.

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Funding

This study was supported by the Russian Science Foundation, project no. 22-24-00401, https://rscf.ru/project/22-24-00401/.

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Authors and Affiliations

  1. Kuban State University, 350040, Krasnodar, Russia

    A. A. Samkov, E. V. Pankratova, M. N. Kruglova, A. V. Bespalov, S. M. Samkova, N. N. Volchenko & A. A. Khudokormov

  2. Sirius University of Science and Technology, 354340, “Sirius” Federal Territory, Krasnodar krai, Russia

    E. V. Pankratova

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  1. A. A. Samkov
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Samkov, A.A., Pankratova, E.V., Kruglova, M.N. et al. Decolorization of Crystal Violet by a Mixed Culture under the Influence of Bioelectrochemical Stimulation. Appl Biochem Microbiol 60, 467–475 (2024). https://doi.org/10.1134/S0003683824603585

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