Abstract
Nonylphenol is an aromatic organic compound that has an estrogen-like effect and has a negative effect on the human endocrine system. A method has been developed for the competitive determination of nonylphenol using magnetic particles, rabbit antiserum, nonylphenol conjugate with soybean trypsin inhibitor (STI) and biotin. The principle of the analysis is the formation of immune complexes on the surface of magnetite particles due to covalent immobilization of protein G through the oriented immobilization of polyclonal antibodies from rabbit serum during a competitive reaction between the free analyte (nonylphenol) and the bound one (as part of the nonylphenol-STI-biotin conjugate) for the binding sites of specific antibodies. The detection of formed immune complexes is proposed to be carried out using a streptavidin-polyperoxidase conjugate, which makes it possible to achieve a nine-fold gain in the level of the analytical signal. The developed ELISA using magnetite particles allows us to achieve a detection limit of nonylphenol at the level of 3.8 ng/mL, which is 14.5 times lower in comparison with the classic competitive ELISA (55 ng/mL). Based on the results of the experimental work, the optimized volume of the test sample was 500 μL, which makes it possible to concentrate low-contaminated samples by 17 times.
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REFERENCES
Evans, A.E.V., Mateo-Sagasta, J., Qadir, M., Boelee, E., and Ippolito, A., Curr. Opin. Environ. Sustain., 2019, vol. 36, pp. 20–27.
Zamora-Ledezma, C., Negrete-Bolagay, D., Figueroa, F., Zamora-Ledezma, E., Ni, M., Alexis, F., and Guerrero, V.H., Environ. Technol. Innov., 2021, vol. 22, p. 101504. https://doi.org/10.1016/j.eti.2021.101504
Fang, W., Peng, Y., Muir, D., Lin, J., and Zhang, X., Environ. Int., 2019, vol. 131, p. 104994. https://doi.org/10.1016/j.envint.2019.104994
Fuller, R., Landrigan, P.J., Balakrishnan, K., Bathan, G., Bose-O', ReillyS., Brauer, M., et al., Lancet Planet. Health, 2022, vol. 6, no. 6, pp. e535–e547.
Palani, G., Arputhalatha, A., Kannan, K., Lakkaboyana, S.K., Hanafiah, M.M., Kumar, V., and Marella, R.K., Molecules, 2021, vol. 26, no. 9, p. 2799. https://doi.org/10.3390/molecules26092799
Babuji, P., Thirumalaisamy, S., Duraisamy, K., and Periyasamy, G., Water, 2023, vol. 15, no. 14, p. 2532. https://doi.org/10.3390/w15142532
Bhandari, G., Bagheri, A.R., Bhatt, P., and Bilal, M., Chemosphere, 2021, vol. 275, p. 130013. https://doi.org/10.1016/j.chemosphere.2021.130013
Gałązka, A. and Jankiewicz, U., Microorganisms, 2022, vol. 10, no. 11, p. 2236. https://doi.org/10.3390/microorganisms10112236
Morin-Crini, N., Lichtfouse, E., Liu, G., Balaram, V., Ribeiro, A.R.L., Lu, Z., et al., Environ. Chem. Lett., 2022, vol. 20, no. 4, pp. 2311–2338.
Chen, Y., Yang, J., Yao, B., Zhi, D., Luo, L., and Zhou, Y., Environ. Pollut., 2022, vol. 310, p. 119918. https://https://doi.org/https://doi.org/10.1016/j.envpol.2022.119918
Hong, Y., Feng, C., Yan, Z., Wang, Y., Liu, D., Liao, W., and Bai, Y., Environ. Chem. Lett., 2020, vol. 18, no. 6, pp. 2095–2106.
Careghini, A., Mastorgio, A.F., Saponaro, S., and Sezenna, E., Environ. Sci. Pollut. Res., 2015, vol. 22, no. 8, pp. 5711–5741.
Jardak, K., Drogui, P., and Daghrir, R., Environ. Sci. Pollut. Res., 2016, vol. 23, no. 4, pp. 3195–3216.
Lu, D., Yu, L., Li, M., Zhai, Q., Tian, F., and Chen, W., Chemosphere, 2021, vol. 275, p. 129973. https://doi.org/10.1016/j.chemosphere.2021.129973
Noorimotlagh, Z., Mirzaee, S.A., Martinez, S.S., Rachon, D., Hoseinzadeh, M., and Jaafarzadeh, N., Environ. Res., 2020, vol. 184, p. 109263. https://doi.org/10.1016/j.envres.2020.109263
Directive 2013/39/eu of the European parliament and of the council of 12 August 2013 amending Directives 2000/60/EC and 2008/105/EC as regards priority substances in the field of water policy.
Shih, H.-K., Shu, T.-Y., Ponnusamy, V.K., and Jen, J.-F., Anal. Chim. Acta, 2015, vol. 854, pp. 70–77.
Vargas-Berrones, K., Díaz de León-Martínez, L., Bernal-Jácome, L., Rodriguez-Aguilar, M., Ávila-Galarza, A., and Flores-Ramírez, R., Talanta, 2020, vol. 209, p. 120546. https://doi.org/10.1016/j.talanta.2019.120546
Aparicio, I., Martín, J., Santos, J.L., Malvar, J.L., and Alonso, E., J. Chromatogr., A, 2017, vol. 1500, pp. 43–52.
Yin, H.-L. and Zhou, T.-N., Chinese J. Anal. Chem, 2022, vol. 50, no. 8, p. 100112. https://https://doi.org/https://doi.org/10.1016/j.cjac.2022.100112
Céspedes, R., Skryjová, K., Raková, M., Zeravik, J., Fránek, M., Lacorte, S., and Barceló, D., Talanta, 2006, vol. 70, no. 4, pp. 745–751.
Matsui, K., Kawaji, I., Utsumi, Y., Ukita, Y., Asano, T., Takeo, M., and Kato, D.-I., and Negoro, S., J. Biosci. Bioeng., 2007, vol. 104, no. 4, pp. 347–350.
Yakovleva, J.N., Lobanova, A.Y., Shutaleva, E.A., Kourkina, M.A., Mart’ianov, A.A., Zherdev, A.V., Dzantiev, B.B., and Eremin, S.A., Anal. Bioanal. Chem., 2004, vol. 378, no. 3, pp. 634–641.
Ermolaeva, T.N., Dergunova, E.S., Kalmykova, E.N., and Eremin, S.A., J. Anal. Chem., 2006, vol. 61, no. 6, pp. 609–613.
Badea, M., Nistor, C., Goda, Y., Fujimoto, S., Dosho, S., Danet, A., Barcelo, D., Ventura, F., and Emneus, J., Analyst, 2003, vol. 128, no. 7, pp. 849–856.
Mart’ianov, A.A., Zherdev, A.V., Eremin, S.A., and Dzantiev, B.B., Int. J. Env. Anal. Chem., 2004, vol. 84, no. 13, pp. 965–978.
Mart’ianov, A.A., Dzantiev, B.B., Zherdev, A.V., Eremin, S.A., Cespedes, R., Petrovic, M., and Barcelo, D., Talanta, 2005, vol. 65, no. 2, pp. 367–374.
Berlina, A.N., Komova, N.S., Serebrennikova, K.V., Zherdev, A.V., and Dzantiev, B.B., Eng. Proc., 2023, vol. 48, no. 1, p. 9. https://doi.org/10.3390/CSAC2023-14919
Berlina, A.N., Ragozina, M.Y., Gusev, D.I., Zherdev, A.V., and Dzantiev, B.B., Chemosensors, 2023, vol. 11, no. 7, p. 393. https://doi.org/10.3390/chemosensors11070393
Kuang, H., Liu, L., Xu, L., Ma, W., Guo, L., Wang, L., and Xu, C., Sensors, 2013, vol. 13, no. 7, pp. 8331–8339.
Kato, M., Ihara, Y., Nakata, E., Miyazawa, M., Sasaki, M., Kodaira, T., and Nakazawa, H., Food Agric. Immunol., 2007, vol. 18, nos. 3–4, pp. 179–187.
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This study was financially supported by the Russian Science Foundation (grant no. 22-13-00293).
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Berlina, A.N., Barshevskaya, L.V., Serebrennikova, K.V. et al. Development of Microplate Immunoenzyme Determination of Nonylphenol with Magnetic Sample Concentration. Appl Biochem Microbiol 60, 496–502 (2024). https://doi.org/10.1134/S0003683824603536
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DOI: https://doi.org/10.1134/S0003683824603536