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Identification, Characterization and Expression of Two Transcription Factors MYB44 and WRKY22 from Drought Tolerant Peanut Cultivar L14

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Abstract

Peanut is an important food crop that provides vegetable oil and protein to the global agricultural economy. However, its productivity is significantly impacted by environmental stresses, particularly drought. A recent study has identified and cloned two transcription factors, AhL14_MYB44 and AhL14_WRKY22, associated with various abiotic stresses. In silico analysis revealed that AhL14_MYB44 had two DNA-binding domains, while AhL14_WRKY22 had a DNA-binding domain involved in regulating plant defense responses. Both proteins were localized in the nucleus, and no transmembrane domains or signal peptides were detected. The study identified that MYB44 and WRKY22 were located on peanut chromosomes 08, 17 and 3, 13, respectively. Further analysis showed that these factors interacted with various abiotic stress-related genes. The DNA-binding domains were modeled, and the docking analysis indicated that both proteins had a high probability of binding to the promoters for regulating drought stress-related genes, such as LEA1 and RD29A. The study conducted in silico analysis and RT-PCR to investigate the expression levels of MYB44 and WRKY22 genes in different organs of peanuts. Their expression was upregulated in root tissues under mannitol stress compared to the control. The study suggests that MYB44 and WRKY22 may play critical roles in the peanut’s response to drought stress, in addition to their other functions, and could offer potential applications in crop improvement strategies aimed at developing transgenic drought-tolerant peanut cultivars.

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REFERENCES

  1. Ojiewo, C.O., Janila, P., Bhatnagar-Mathur, P., Pandey, M.K., Desmae, H., Okori, P., et al., Front. Plant Sci., 2020, vol. 11, p. 29. https://doi.org/10.3389/fpls.2020.00029

    Article  PubMed  PubMed Central  Google Scholar 

  2. Genomic Designing of Climate-Smart Oilseed Crops, Kole, C., Ed., Springer, 2019, pp. 133–172. https://doi.org/10.1007/978-3-319-93536-2_3

  3. Upadhyaya, H.D., Dwivedi, S.L., Vadez, V., Hamidou, F., Singh, S., Varshney, R.K., et al., Crop Sci., 2014, vol. 54, no. 2, pp. 679–693. https://doi.org/10.2135/cropsci2013.07.0493

    Article  Google Scholar 

  4. Legumes: Physiology and Molecular Biology of Abiotic Stress Tolerance, Samy, P.M.A., Ramasamy, A.V. C., and Kumar, B.S., Singapore: Springer Nature Singapore, 2023, pp. 279–290. https://doi.org/10.1007/978-981-19-5817-5_11

  5. Zhao, N., He, M., Li, L., Cui, S., Hou, M., Wang, L., et al., PLoS One, 2020, vol. 15, no. 4, p. e0231396. https://doi.org/10.1371/journal.pone.0231396

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Tang, Y., Du, G., Xiang, J., Hu, C., Li, X., Wang, W., et al., Genomics, 2022, vol. 114, no. 1, pp. 171-184. https://doi.org/10.1016/j.ygeno.2021.12.015

    Article  CAS  PubMed  Google Scholar 

  7. Chen, N., Yang, Q., Pan, L., Chi, X., Chen, M., Hu, D., et al., Gene, 2014, vol. 533, no. 1, pp. 332–345. https://doi.org/10.1016/j.gene.2013.08.092

    Article  CAS  PubMed  Google Scholar 

  8. Kakeshpour, T., Nayebi, S., Rashidi Monfared, S., Moieni, A., and Karimzadeh, G., Physiol. Mol. Biol., 2015, vol. 21, pp. 465–478. https://doi.org/10.1007/s12298-015-0325-z

    Article  CAS  Google Scholar 

  9. Song, H., Wang, P., Lin, J.Y., Zhao, C., Bi, Y., and Wang, X., Front. Plant Sci., 2016, vol. 7, p. 534. https://doi.org/10.3389/fpls.2016.00534

  10. Erpen, L., Devi, H.S., Grosser, J.W., and Dutt, M., Plant Cell Tiss. Organ Cult., 2018, vol. 132, pp. 1–25. https://doi.org/10.1007/s11240-017-1320-6

    Article  CAS  Google Scholar 

  11. Wang, X., Niu, Y., and Zheng, Y., Int. J. Mol. Sci., 2021, vol. 22, no. 11, p. 6125. https://doi.org/10.3390/ijms22116125

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Wang, S., Xu, Z., Yang, Y., Ren, W., Fang, J., and Wan, L., Front. Plant Sci., 2023, vol. 14, p. 77. https://doi.org/10.3389/fpls.2023.1102174

    Article  Google Scholar 

  13. Hu, W., Ren, Q., Chen, Y., Xu, G., and Qian, Y., BMC Plant Biol., 2021, vol. 21, pp. 1–21.

    Article  Google Scholar 

  14. Stracke, R., Werber, M., and Weisshaar, B., Curr. Opin. Plant Biol., 2001, vol. 4, no. 5, pp. 447–456. https://doi.org/10.1016/S1369-5266(00)00199-0

    Article  CAS  PubMed  Google Scholar 

  15. Shim, J.S., Jung, C., Lee, S., Min, K., Lee, Y.W., Choi, Y., et al., Plant J., 2013, vol. 73, no. 3, pp. 483–495. https://doi.org/10.1111/tpj.12051

    Article  CAS  PubMed  Google Scholar 

  16. Persak, H. and Pitzschke, A., Int. J. Mol. Sci., 2014, vol. 15, no. 2, pp. 2517–2537. https://doi.org/10.3390/ijms15022517

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Jaradat, M.R., Feurtado, J.A., Huang, D., Lu, Y., and Cutler, A.J., BMC Plant Biol., 2013, vol. 13, pp. 1–19. https://doi.org/10.1186/1471-2229-13-192

    Article  CAS  Google Scholar 

  18. Shamloo-Dashtpagerdi, R., Razi, H., Ebrahimie, E., and Niazi, A., Mol. Biol. Rep., 2018, vol. 45, pp. 1111–1124. https://doi.org/10.1007/s11033-018-4262-0

    Article  CAS  PubMed  Google Scholar 

  19. Jung, C., Seo, J.S., Han, S.W., Koo, Y.J., Kim, C.H., Song, S.I., et al., Plant Physiol., 2008, vol. 146, no. 2, pp. 623–635. https://doi.org/10.1104/pp.107.110981

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Seo, J.S., Sohn, H.B., Noh, K., Jung, C., An, J.H., Donovan, C.M., et al., Mol. Breed., 2012, vol. 29, pp. 601–608. https://doi.org/10.1007/s11032-011-9576-8

    Article  CAS  Google Scholar 

  21. Yang, A., Dai, X., and Zhang, W.H., J. Exp. Bot., 2012, vol. 63, no. 7, pp. 2541–2556. https://doi.org/10.1093/jxb/err431

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Qiu, Z., Yan, S., Xia, B., Jiang, J., Yu, B., Lei, J., et al., J. Exp. Bot., 2019, vol. 70, no. 19, pp. 5343–5354. https://doi.org/10.1093/jxb/erz259

    Article  CAS  PubMed  Google Scholar 

  23. Kloth, K.J., Wiegers, G.L., Busscher-Lange, J., van Haarst, J.C., Kruijer, W., Bouwmeester, H.J., et al., J. Exp. Bot., 2016, vol. 67, no. 11, pp. 3383–3396. https://doi.org/10.1093/jxb/erw159

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Hsu, F.C., Chou, M.Y., Chou, S.J., Li, Y.R., Peng, H.P., et al., Plant Cell, 2013, vol. 25, no. 7, pp. 2699–2713. https://doi.org/10.1105/tpc.113.114447

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Long, Q., Du, M., Long, J., Xie, Y., Zhang, J., Xu, L., et al., Hortic. Res., 2021, vol. 8, p. 50. https://doi.org/10.1038/s41438-021-00486-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Li, T., Zhou, T., Liang, J., Zhang, D., Teng, N., et al., Ornam. Plant Res., 2022, vol. 2, no. 1, pp. 1–10. https://doi.org/10.48130/OPR-2022-0017

    Article  CAS  Google Scholar 

  27. Zhuang, W., Chen, H., Yang, M., Wang, J., Pandey, M.K., Zhang, C., et al., Nat. Genet., 2019, vol. 51, no. 5, pp. 865–876. https://doi.org/10.1038/s41588-019-0402-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Chi, X., Hu, R., Yang, Q., Zhang, X., Pan, L., Chen, N., et al., Mol. Genet. Genom., 2012, vol. 287, pp. 167–176. https://doi.org/10.1007/s00438-011-0665-5

    Article  CAS  Google Scholar 

  29. Divi, U.K., Rahman, T., and Krishna, P., BMC Plant Biol., 2010, vol. 10, pp. 1–14. https://doi.org/10.1186/1471-2229-10-151

    Article  CAS  Google Scholar 

  30. Wang, J., Song, L., Gong, X., Xu, J., and Li, M., Int. J. Mol. Sci., 2020, vol. 21, no. 4, p. 1446. https://doi.org/10.3390/ijms21041446

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Zhang, N., Han, L., Xu, L., and Zhang, X., Horttechnology, 2018, vol. 28, no. 3, pp. 319–326. https://doi.org/10.21273/HORTTECH03976-18

    Article  CAS  Google Scholar 

  32. Xu, C., Zhang, Y.P., and Yang, H.B., Chil. J. Agric. Res., 2022, vol. 82, no. 2, pp. 234–243. https://doi.org/10.4067/S0718-58392022000200234

    Article  Google Scholar 

  33. Li, H., Guo, Y., Lan, Z., Xu, K., Chang, J., Ahammed, G. J., et al., Hortic. Res., 2021, vol. 8, p. 57. https://doi.org/10.1038/s41438-021-00496-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Vishwakarma, K., Upadhyay, N., Kumar, N., Yadav, G., Singh, J., Mishra, R. K., et al., Front. Plant Sci., 2017, vol. 8, p. 161. https://doi.org/10.3389/fpls.2017.00161

    Article  PubMed  PubMed Central  Google Scholar 

  35. Soumya, P.R., Kumar, P., and Pal, M., Ind. J. Plant Physiol., 2017, vol. 22, pp. 267–278. https://doi.org/10.1007/s40502-017-0316-x

    Article  CAS  Google Scholar 

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Funding

This study was supported by Vietnam Ministry of Education and Training (code: CT-2021-01-DHH-04).

The authors thank the partial support of Hue University under the Core Research Program, Grant no. NCM.DHH.2022.13.

Hoang Kha received a scholarship from the Master's Program of the Vingroup Innovation Foundation (VINIF), code VINIF.2023.ThS.064.

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Correspondence to N. H. Loc.

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Tien, N.Q., Kha, H. & Loc, N.H. Identification, Characterization and Expression of Two Transcription Factors MYB44 and WRKY22 from Drought Tolerant Peanut Cultivar L14. Appl Biochem Microbiol 60, 26–37 (2024). https://doi.org/10.1134/S0003683824010174

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