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OmpA is Involved in the Early Response of Escherichia coli to Antibiotics as a Hub Outer Membrane Protein

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Abstract

The environmental pollution caused by antibiotics is becoming more and more serious. Outer membrane proteins (OMPs) play key roles in the resistance of bacteria to antibiotics. Transcriptomic profiles of Esherichia coli treated with different types of antibiotics revealed the pathways and response mechanisms of OMP-related genes up- or down-regulated following antibiotics treatment. Among all the 4497 genes, 73 OMPs were screened. In gentamicin- or ampicillin-treated groups, 51 or 23 genes were differentially expressed, respectively. The results of protein-protein interaction networks showed that ompA, chiP, phoE, and cusC were overlapped hub genes in both groups. Considering the scores of nodes calculated by cytoHubba, OmpA was the key one among the OMPs through the calculation of Cytoscape. Finally, the knockout and overexpression mutants and complementation strain of ompA were constructed. The results of drug resistance to 4 types of antibiotics analysis confirmed the role of ompA in the resistance of E. coli to antibiotics. This work provides direct evidence for the role of outer membrane proteins, especially OmpA, in drug resistance of E. coli to different types of antibiotics. The findings in this study can lead to new strategies for improving the effectiveness of antibiotics.

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DATA AVAILABILITY

All data and materials used in this research are publicly available. Raw sequence data from this study have been submitted to the NCBI sequence read archive under the BioProject accession [PRJNA510855] and available at the following link: https://trace.ncbi.nlm.nih.gov/Traces/sra_sub/sub.cgi?acc=SRP173974. Other supporting data are included as additional files listed below and submitted with the manuscript.

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ACKNOWLEDGMENTS

We wish to thank Prof. J.A. Imlay for providing wildtype strain MG1655 for this work. We are grateful for the free online platform of Majorbio I-Sanger Cloud Platform (www.i-sanger.com). We would also like to thank English Editing by Elsevier Language Editing Services (Registration no. 331566771). This work was supported by the Natural Science.

Funding

Foundation of Guangdong Province (no. 2019A1515011685) received by Prof. Z. Lu, and Science and Technique Foundation of Henan Province (202102110296) received by Prof. X. Li.

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Authors and Affiliations

  1. College of Food and Bioengineering, Henan University of Science and Technology, 471023, Luoyang, China

    Q. Zhao, Y. Wu, B. Li, L. He, J. Sun, S. Gu & X. Li

  2. College of Medical Technology and Engineering, Henan University of Science and Technology, 471023, Luoyang, China

    X. Pang

  3. Key Laboratory of Microbial Resources Exploitation and Utilization, 471023, Luoyang, China

    Y. Wu, S. Gu & X. Li

  4. National Demonstration Center for Experimental Food Processing and Safety Education, 471000, Luoyang, China

    X. Li

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Correspondence to X. Pang, S. Gu or X. Li.

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Zhao, Q., Wu, Y., Li, B. et al. OmpA is Involved in the Early Response of Escherichia coli to Antibiotics as a Hub Outer Membrane Protein. Appl Biochem Microbiol 59, 608–621 (2023). https://doi.org/10.1134/S0003683823050204

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