Abstract
The production of diadinoxanthin and its cytostatic activity in an intensive culture of C. closterium has been estimated via molecular modeling and experiments with the human tumor cell cultures OVCAR5, OVCAR8, KURAMOCHI, and OVSAHO. According to data from both the simulation and experiments with the cell cultures, diadinoxanthin demonstrates lower activity (by more than four times) in comparison with fucoxanthin. The IC50 value for diadinoxanthin is achieved at a concentration of more than 100 μM, while fucoxanthin exhibits a cytostatic effect below 18.75 μM. In a flow-through culture of C. closterium, the production of diadinoxanthin can exceed the production of fucoxanthin by 14 times, which can make the technology for its production less expensive and can significantly reduce the cost of therapeutic and prophylactic drugs based on microalgae.
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ACKNOWLEDGMENTS
The authors are grateful to E.A. Akimov (Institute for Molecular Medicine Finland, HiLIFE) and Doctor of Philosophy T. Maoka (Research Institute for Production Development, Kyoto, Japan) for their help in the experimental work with cell cultures and the identification of carotenoids.
Funding
The work was carried out within the framework of the state assignment of the InBYuM Federal Research Center on the topic “Study of mechanisms to control production processes in biotechnological complexes in order to develop scientific foundations to obtain biologically active substances and technical products of marine genesis” (State registration no. 121030300149-0) and the state assignment of the Ministry of Education and Science of the Russian Federation within the framework of the project for the creation and development of world-class research centers, “Digital biodesign and personalized healthcare” (State registration no. 075-15-2020-926).
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Gevorgiz, R.G., Gureev, M.A., Zheleznova, S.N. et al. Production of Diadinoxanthin in an Intensive Culture of the Diatomaceous Alga Cylindrotheca closterium (Ehrenb.) Reimann et Lewin. and Its Proapoptotic Activity. Appl Biochem Microbiol 58, 261–268 (2022). https://doi.org/10.1134/S0003683822010033
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DOI: https://doi.org/10.1134/S0003683822010033