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Development of Immunochromatographic Test System for Detection of Antibiotic Clinafloxacin and Its Application for Honey Control

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Abstract

To ensure express control of food quality and safety, we developed an immunochromatographic test system for detection of the antibiotic clinafloxacin. The test system implements an indirect competitive immunoassay format based on the interaction of unmodified specific polyclonal antibodies with the antibiotic potentially present in the sample and the antibiotic–protein conjugate immobilized on the working membrane of the test strip. The detection of immune complexes formed on the membrane is carried out using a conjugate of anti-species antibodies and gold nanoparticles. We determined immunochromatography conditions that ensure the achievement of the minimum detection limit. At the assay duration of 20 min, the instrumental and visual detection limits of clinafloxacin comprise 0.3 and 10 ng/mL, respectively. The selectivity of the test system in relation to antibiotics from the class of fluoroquinolones was studied. To ensure the control of the contamination of honey with clinafloxacin and structurally related fluoroquinolones, we identified an optimal sample preparation regimen and confirmed the efficacy of the developed test system.

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The authors are grateful to A. A. Ryazanov (Research Center of Biotechnology of RAS) for his help in characterizing the specificity of the test system.

Funding

This work was financially supported by the Russian Science Foundation (project no. 20-76-10033).

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia

    E. A. Zvereva, D. V. Sotnikov, K. A. Belichenko, O. D. Hendrickson, I. A. Shanin, A. V. Zherdev & B. B. Dzantiev

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  1. E. A. Zvereva
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  2. D. V. Sotnikov
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  3. K. A. Belichenko
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  4. O. D. Hendrickson
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  5. I. A. Shanin
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  6. A. V. Zherdev
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  7. B. B. Dzantiev
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Correspondence to B. B. Dzantiev.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Zvereva, E.A., Sotnikov, D.V., Belichenko, K.A. et al. Development of Immunochromatographic Test System for Detection of Antibiotic Clinafloxacin and Its Application for Honey Control. Appl Biochem Microbiol 57, 778–785 (2021). https://doi.org/10.1134/S0003683821060144

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  • DOI: https://doi.org/10.1134/S0003683821060144

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