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Activity of Methanogenic Archaea during the Composting of Organic Waste

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Abstract

The composting of a mixture of excess sludge from biological treatment facilities for wastewater from dairy production and vegetable and wood waste in a ratio of 3 : 3 : 4 (by volume) with a high amount of dry substance (27.5%) at extremely high temperatures (75–82°C) made it possible to isolate active mesophilic and thermophilic methanogenic consortia. The dynamics of methane formation upon the liquid-phase fermentation of the studied mixture was studied. Accelerated methanogenesis and the highest methane yield (31.7 ± 2.9 mM L–1) were observed with culturing at 55°C. Analysis of the volatile fatty-acid production with liquid-phase fermentation showed that their content was low (<10 mM L–1). This amount could not inhibit methanogenesis, regardless of the applied substrates and temperature conditions. The 16S rRNA sequencing of the obtained consortia showed the presence of methanogenic archaea Methanosarcina thermophila, Methanothermobacter thermoautotrophicus, Candidatus ‘Methanogranum caenicola’, and Methanofollis ethanolicus. The consortia also included archaea that represent putative new taxa that are phylogenetically close to Candidatus ‘Methanoplasma termitum’ and Methanomassiliicoccus luminyensis. Of the mentioned methanogenic archaea, the latter four were found in composting organic waste for the first time. The obtained consortia can conduct active methanogenesis and can be applied as an inoculum in the anaerobic treatment of organic wastes, including composting wastes, in order to intensify and increase the biogas yield.

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ACKNOWLEDGMENTS

The work was carried out on the scientific equipment of the Bioengineering Core Facility (Biotechnology Research Center of the Russian Academy of Sciences).

Funding

This work was supported by the Russian Foundation for Basic Research, project no. mk-18-29-25035.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071, Moscow, Russia

    V. V. Mironov, V. V. Potokina, E. A. Botchkova, A. V. Vanteeva & S. N. Parshina

  2. Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071, Moscow, Russia

    N. A. Zagustina

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  1. V. V. Mironov
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Mironov, V.V., Potokina, V.V., Botchkova, E.A. et al. Activity of Methanogenic Archaea during the Composting of Organic Waste. Appl Biochem Microbiol 57, 750–759 (2021). https://doi.org/10.1134/S0003683821060107

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