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In Vitro Assessment of Probiotic Potential and Functional Properties of Lactobacillus reuteri LR1

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Abstract

Research on the effects of various antimicrobial agents on the strain Lactobacillus reuteri LR1 indicated its susceptibility to lincomycin, amoxicillin, and chloramphenicol, as well as resistance to other studied antibiotics. Upon milk fermentation by L. reuteri LR1 for 24 h, there was a significant increase in antioxidant and ACE-inhibitory activities, along with a decrease in L-leucine equivalents, as compared to the original milk. Further cultivation resulted in an increase in the inhibitory activity of the proteolytic, antioxidant, and angiotensin-converting enzyme (ACE), reaching its highest value after 96 h. HPLC-MS/MS analysis of the peptide profile of milk fermented by the lactobacillus showed the presence of peptides possessing ACE-inhibitory, antimicrobial, antioxidant, and immunomodulatory activities. Analysis of the substrate specificity of specificity of L. reuteri LR1 CEP proteinase toward the (f1-23) αs1-casein fragment revealed its unique specificity, which may indicate the PI/PIII type.

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ACKNOWLEDGMENTS

The equipment of the Industrial Biotechnology Center for Collective Use ( Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences) was used in this research.

Funding

The determination of the proteolytic, antioxidant, and ACE-inhibitory activities of the strain L. reuteri LR1, as well as its peptide profile, in this study was supported by the Russian Science Foundation, project no. 16-16-00094.

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Authors and Affiliations

  1. All-Russia Scientific Research Institute of the Dairy Industry, 115093, Moscow, Russia

    A. V. Begunova, I. V. Rozhkova & Yu. I. Krysanova

  2. Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 119071, Moscow, Russia

    O. S. Savinova & T. V. Fedorova

Authors
  1. A. V. Begunova
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  2. O. S. Savinova
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  3. I. V. Rozhkova
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Correspondence to T. V. Fedorova.

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The authors declare no conflict of interest. This article does not contain any studies involving animals or human participants performed by the authors.

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Translated by A. Panyushkina

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Begunova, A.V., Savinova, O.S., Rozhkova, I.V. et al. In Vitro Assessment of Probiotic Potential and Functional Properties of Lactobacillus reuteri LR1. Appl Biochem Microbiol 56, 544–552 (2020). https://doi.org/10.1134/S000368382005004X

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