Antimicrobic Activity of the Lipopeptide Emericellipsin A Isolated from Emericellopsis alkalina against Biofilm-Forming Bacteria


The antimicrobial activity of the new nonribosomal peptide emericellipsin A against biofilm-forming, pathogenic, gram-positive and gram-negative clinical bacteria was studied. It was shown for the first time that peptides from the group of peptaibols can inhibit pathogenic forms of bacteria via their effect on biofilm formation. The most pronounced effect was demonstrated in relation to gram-positive bacteria.

This is a preview of subscription content, access via your institution.

Fig. 1.
Fig. 2.


  1. 1

    Yazici, A., Ortucu, S., Taskin, M., and Marinelli, L., Curr. Top. Med. Chem., 2018, vol. 18, no. 24, pp. 1–6.

    Article  Google Scholar 

  2. 2

    Do, T., Devine, D., and Marsh, P.D., Clin. Cosmet. Invest. Dent., 2013, vol. 5, no. 6 P, pp. 11–19.

  3. 3

    Baranova, A.A., Georgieva, M.L., Bilanenko, E.N., Andreev, Y.A., Rogozhin, E.A., and Sadykova, V.S., Appl. Biochem. Microbiol., 2017, vol. 53, no. 6, pp. 703–710.

  4. 4

    Athina, A., Molchanova, N., and Jenssen, H., Biomolecules, 2018, vol. 8, no. 27, pp. 1–29.

    Article  Google Scholar 

  5. 5

    Costerton, W., Veeh, R., Shirtliff, M., Pasmore, M., Post, C., and Ehrlich, G., J. Clin. Invest., 2003, vol. 112, no. 10, pp. 1466–1477.

    CAS  Article  Google Scholar 

  6. 6

    Fan, L., Sun, J., Zhou, M., Zhou, J., Lao, X., Zheng, H., and Xu, H., Sci. Rep., 2016, vol. 6, no. 24482.

  7. 7

    Jacobsen, A.S. and Jenssen, H., Future Med. Chem., 2012, no. 4, pp. P. 1587–1599.

  8. 8

    Overhage, J., Campisano, A., Bains, M., Torfs, E.C.W., Rehm, B.H.A., and Hancock, R.E.W., Infect. Immun., 2008, vol. 76, no. 9, pp. 4176–4182.

    CAS  Article  Google Scholar 

  9. 9

    Zhurlov, O.S., Uspekhi Sovremennogo estestvoznaniya, 2015, vol. 1, no. 9, pp. 107–109.

  10. 10

    Mansour, S.C., de la Fuente-Núñez, C., and Hancock, R.E.W., J. Pept. Sci., 2015, vol. 21, pp. 323–329.

    CAS  Article  Google Scholar 

  11. 11

    Grum-Grzhimaylo, A.A., Georgieva, M.L., Debets, A.J.M., and Bilanenko, E.N., IMA Fungus, 2013, vol. 4, no. 2, pp. 213–228.

    Article  Google Scholar 

  12. 12

    Rogozhin, E.A., Sadykova, V.S., Baranova, A.A., Vasilchenko, A.S., Lushpa, V.A., Mineev, K.S., Georgieva, M.L., Kul’ko, A.B., Krasheninnikov, M.E., Lyundup, A.V., Vasilchenko, A.V., and Andreev, Y.A., Molecules, 2018, vol. 23, no. 2785.

    Article  Google Scholar 

  13. 13

    Rogozhin, E. and Sadykova, V.A., Proceedlings, 2019, vol. 22, no. 4, pp. 1–12.

    Google Scholar 

  14. 14

    Baranova, A.A., Rogozhin, E.A., Georgieva, M.L., Bilanenko, E.N., Kul’ko, A.B., Yakushev, A.V., Alferova, V.A., and Sadykova, V.S., Appl. Biochem. Microbiol., 2019, vol. 55, no. 2, pp. P. 145–151.

  15. 15

    Sadykova, V.S., Rogozhin, E.A., Baranova, A.A., Georgieva, M.L., Bilanenko, E.N., and Vasil’chenko, A.S., RF Patent No. 2704421, 2019.

  16. 16

    Plakunov, V.K., Martyanov, S.V., Teteneva, N.A., and Zhurina, M.V., Microbiology, 2016, vol. 85, no. 4, pp. 509–513.

    CAS  Article  Google Scholar 

  17. 17

    MBEC™ Assay For High-Throughput Antimicrobial Susceptibility Testing of Biofilms Procedural manual. Version 1.1 For High-Throughput Antimicrobial Susceptibility Testing of Biofilms, ©Innovotech, Inc., 2015.

  18. 18

    O’Toole, G.A., J. Visualized Exp., 2011, vol. 47, p. e2437.

    Article  Google Scholar 

  19. 19

    Trukhacheva, N.V., Matematicheskaya Statistika v mediko-biologicheskikh issledovaniyakh s primeneniem paketa Statistica (Mathematical Statistics in Biomedical Research using the Statistica Package), GEOTAR-Media, 2012.

  20. 20

    Pfalzgraff, A., Brandenburg, K., and Weindl, G., Front. Pharmacol., 2018, vol. 28, no. 9, p. 281.

    CAS  Article  Google Scholar 

  21. 21

    Khan, F., Nguyen, P.D.T., Oloketuyi, S.F., and Kim, Y.M., Curr. Pharm. Biotechnol., 2019, vol. 30, pp. 345–360.

    CAS  Article  Google Scholar 

Download references


The authors are grateful to the staff of the Department of Microbiology and Infectious Diseases of the Orenburg State Agrarian University and personally to M.V. Sycheva for her assistance with the study of the antimicrobial properties of emericellipsin A.


The work on the isolation and accumulation of emericellipsin A for biological testing was carried out with the financial support of the Russian Science Foundation (grant no. 18-74-10073), and the producer was cultivated the financial support of the Russian Federal Property Fund in the framework of scientific project no. 19-34-90088.

Author information



Corresponding author

Correspondence to V. S. Sadykova.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sadykova, V.S., Gavryushina, I.A., Kuvarina, A.E. et al. Antimicrobic Activity of the Lipopeptide Emericellipsin A Isolated from Emericellopsis alkalina against Biofilm-Forming Bacteria. Appl Biochem Microbiol 56, 292–297 (2020).

Download citation


  • biofilm-forming bacteria
  • antimicrobial peptides
  • emericellipsin A
  • alkaliphilic fungi
  • inhibition of formation biofilm