Abstract
In the present study, the mechanisms of Aspergillus niger AM1 and AM2 resistance to white phosphorus were studied. It was shown that the presence of white phosphorus (P4) at a concentration of 0.25% in the medium had a marginal impact on the ratio of living to dead cells during fungal cultivation, which indicates a high resistance of the strains to P4. Observations made with electron microscopy showed an increase in the thickness of the fungal cell wall, which is a barrier to the penetration of white phosphorus. MALDI results revealed the biosynthesis of new protein enzymes that could potentially participate in the neutralization of white phosphorus. In addition, white phosphorus caused activation of the metabolism, accompanied by an increase in the number of mitochondria in the cells.
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REFERENCES
Duerksen-Hughes, P., Richter, P., Ingerman, L., Ruoff, W., Thampi, S., and Donkin, S., Toxicological Profile for White Phosphorus, U.S. Department of Health and Human Services, USA, 1997.
Gleason, W., JOM, 2007, vol. 59, no. 6, pp. 17–19.
Walsh, M.E., Collins, C.M., and Racine, C., Persistence of White Phosphorus Particles in Sediment, Hanover, New Hampshire: US Army Cold Regions Research and Engineering Lab, 1995.
Cummins, C.C., Daedalus, 2014, vol. 143, no. 4, pp. 9–20.
Khomenkov, V.G., Shevelev, A.B., Zhukov, V.G., Zagustina, N.A., Bezborodov, A.M., and Popov, V.O., Appl. Biochem. Microbiol., 2008, vol. 44, no. 2, pp. 133–152.
Mindubaev, A.Z., Ekol. Vestn. Sev. Kavkaza, 2018, vol. 14, no. 1, pp. 11–23.
Singh, B.K. and Walker, A., FEMS Microbiol. Rev., 2006, vol. 30, no. 3, pp. 428–471.
Mindubaev, A.Z., Voloshina, A.D., Babynin, E.V., Badeeva, E.K., Khayarov, Kh.R., Minzanova, S.T., and Yakhvarov, D.G., Ekol. Prom. Rossii, 2018, vol. 22, no. 1, pp. 33–37.
Mindubaev, A.Z., Babynin, E.V., Badeeva, E.K., Piskunov, D.B., Makhiyanov, A.N., Minzanova, S.T., Mironova, L.G., and Voloshina, A.D., Izv. Vuzov,Prikl. Khim. Biotekhnol., 2019, vol. 9, no. 1, pp. 81–94.
Mindubaev, A.Z., Voloshina, A.D., and Akosah, Y.A., Ekol. Vestn. Sev. Kavkaza, 2019, vol. 15, no. 1, pp. 51–57.
Mindubaev, A.Z., Voloshina, A.D., Badeeva, E.K., Khayarov, Kh.R., and Yakhvarov, D.G., Ross. Zh. Prikl. Ekol., 2018, vol. 1, no. 13, pp. 28–32.
Gorshkov, V., Petrova, O., Gogoleva, N., and Gogolev, Y., FEMS Immunol. Med. Microbiol., 2010, vol. 59, no. 3, pp. P. 378–385.
Biserova, N.M., Metody vizualizatsii biologicheskikh ul’trastruktur. Prakticheskoe rukovodstvo dlya biologov (Methods for Visualization of Biological Ultrastructures: A Practical Guide for Biologists), Moscow: KMK, 2013.
Reynolds, E.S., J. Cell Biol., 1963, vol. 17, no. 1, pp. 208–212.
Bhadauria, V. and Peng, Y.-L., J. Microbiol., 2010, vol. 50, no. 1, pp. 127–S131.
The bicinchoninic acid (BCA) assay for protein quantitation, in The Protein Protocols Handbook, 2nd ed., Walker, J.M., Ed., Totowa, New Jersey: Humana Press, 2002, pp. 11–14.
Lelong, C., Chevallet, M., Luche, S., and Rabilloud, T., Methods Mol. Biol., 2009, vol. 519, pp. 339–350.
Kang, X., Kirui, A., Muszynski, A., Widanage, M.C.D., Chen, A., Azadi, P., Wang, P., Mentink-Vigier, F., and Wang, T., Nat. Commun., 2018, vol. 9, no. 2747, pp. 1–12.
Mindubaev, A.Z., Voloshina, A.D., Khayarov, Kh.R., Sakhapov, I.F., Badeeva, E.K., Strobykina, A.S., Validov, Sh.Z., Babaev, V.M., Minzanova, S.T., Mironova, L.G., Akosah, Y.A., and Yakhvarov, D.G., Butlerov. Soobshch., 2017, vol. 51, no. 8, pp. 1–26.
Adams, N.E., Thiaville, J.J., Proestos, J., Juarez-Vazquez, A.L., McCoy, A.J., Barona-Gómez, F., Iwata-Reuyl, D., de Crécy-Lagard, V., and Maurelli, A.T., MBio, 2014, vol. 5, no. 4, pp. 1–14. e01378-14.
Maita, N., Okada, K., Hatakeyama, K., and Hakoshima, T., PNA, vol. 99, no. 3, pp. 1212–1217.
Harriff, M.J., Danelishvili, L., Wu, M., Wilder, C., McNamara, M., Kent, M.L., and Bermudez, L.E., J. Bacteriol., 2009, vol. 191, no. 4, pp. 1132–1142.
Mindubaev, A.Z., Babynin, E.V., Badeeva, E.K., Kabirova, G.K., Sinitsina, A.A., Shaikhutdinov, R.K., Sharipov, A.A., Minzanova, S.T., Mironova, L.G., and Akosah, Y.A., Butlerov. Soobshch., 2018, vol. 56, no. 11, pp. 1–24.
ACKNOWLEDGMENTS
Laser confocal microscopy and transmission electron microscopy were carried out at the Analytical Microscopy KFU Interdisciplinary Center. Mass spectrometric studies were carried out at the Center for Collective Use of the Kazan Scientific Center, Federal Research Center, Russian Academy of Sciences.
Funding
The authors are grateful to the Innovation Support Fund for financial support (project no. 39113 (C1-34299)).
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Mindubaev, A.Z., Kuznetsova, S.V., Evtyugin, V.G. et al. Effect of White Phosphorus on the Survival, Cellular Morphology, and Proteome of Aspergillus niger. Appl Biochem Microbiol 56, 194–201 (2020). https://doi.org/10.1134/S0003683820020118
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DOI: https://doi.org/10.1134/S0003683820020118